PDGF-B Antibody

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ProSci
Product Code: PSI-7199
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7199-0.02mg0.02mg£150.00
Quantity:
PSI-7199-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of PDGF-B in rat liver tissue lysate with PDGF-B antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of PDGF-B in human liver tissue with PDGF-B antibody at 20 μg/ml.
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Western blot analysis of PDGF-B in rat liver tissue lysate with PDGF-B antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of PDGF-B in human liver tissue with PDGF-B antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
PDGF-B Antibody: SIS, SSV, IBGC5, PDGF2, c-sis, PDGF-2, SIS, Platelet-derived growth factor subunit B, PDGF subunit B
Application Note:
PDGF-B antibody can be used for detection of PDGF-B by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
PDGF-B Antibody: The platelet-derived growth factor beta (PDGF-B) is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB). Mutations in this gene are associated with meningioma , and reciprocal translocations between chromosomes 22 and 7, at sites where the PDGF-B gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans.
Background References:
  • Johnsson A, Heldin CH, Wasteson A, et al. The c-sis gene encodes a precursor of the B chain of platelet-derived growth factor. EMBO J. 1984; 3:921-8.
  • Fredriksson L, Li H, and Eriksson U. The PDGF family: four gene products for five dimeric isoforms. Cyto. Growth Factor Rev. 2004; 15:197-204.
  • Maxwell M, Galanopoulos T, Hedley-Whyte ET, et al. Human meningiomas co-express platelet-derived growth factor )PDGF) and PDGF-receptor genes and their protein products. Int. J. Cancer 1990; 46:16-21.
  • Simon MP, Navarro M, Roux D, et al. Structural and functional analysis of a chimeric protein COL1A1-PDGFB generated by the translocation t(17;22)(q22;q13.1) in Dermatofibrosarcoma protuberans (DP). Oncogene 2001; 20:2965-75.
Buffer:
PDGF-B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (100%)
Immunogen:
Rabbit polyclonal PDGF-B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PDGF-B.

The immunogen is located within the last 50 amino acids of PDGF-B.
NCBI Gene ID #:
5155
NCBI Official Name:
platelet-derived growth factor beta polypeptid
NCBI Official Symbol:
PDGFB
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 27 kDa
Protein Accession #:
NP_002599
Protein GI Number:
4505681
Purification:
PDGF-B Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
P01127
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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