CCL2 Antibody

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ProSci
Product Code: PSI-7201
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7201-0.02mg0.02mg£150.00
Quantity:
PSI-7201-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of CCL2 in K562 cell lysate with CCL2 antibody at 1 μg/mL.
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Immunohistochemistry of CCL2 in human spleen tissue with CCL2 antibody at 5 μg/ml.
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Immunofluorescence of CCL2 in human spleen tissue with CCL2 antibody at 20 μg/ml.
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Western blot analysis of CCL2 in K562 cell lysate with CCL2 antibody at 1 μg/mL.
Immunohistochemistry of CCL2 in human spleen tissue with CCL2 antibody at 5 μg/ml.
Immunofluorescence of CCL2 in human spleen tissue with CCL2 antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
CCL2 Antibody: HC11, MCAF, MCP1, MCP-1, SCYA2, GDCF-2, SMC-CF, HSMCR30, C-C motif chemokine 2, HC11
Application Note:
CCL2 antibody can be used for detection of CCL2 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
CCL2 Antibody: CCL2, also known as monocyte chemotactic protein 1 (MCP1), belongs to the intercrine beta (chemokine CC) family. It is produced by a variety of cell types and is a potent chemoattractant for monocytes, memory T lymphocytes, and natural killer (NK) cells. It is upregulated during infection and inflammation. CCL2 is a potent basophil activator but does not affect eosinophils, whereas the related protein MCP2 stimulates both eosinophils and basophils. MCP3 has been shown to have the broadest range of influence. CCL2 has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis.
Background References:
  • Carr MW, Roth SJ, Luther E, et al. Monocyte chemoattractant protein 1 acts as a T-lymphocyte chemoattractant. Proc. Natl. Acad. Sci. USA 1994; 91:3652-6.
  • Conductier G, Blondeau N, Guyon A, et al. The role of monocyte chemoattractant protein MCP1/CCL2 in neuroinflammatory diseases. J. Neuroimmunol. 2010; 224:93-100.
  • Taub DD, Proost P, Murphy WJ, et al. Monocyte chemotactic protein-1 (MCP-1), -2, and -3 are chemotactic for human T lymphocytes. J. Clin. Invest. 1995; 95:1370-6
  • Bandinelli F, Del Rosso A, Gabrielli A, et al. CCL2, CCL3 and CCL5 chemokines in systemic sclerosis: the correlation with SSc clinical features and the effect of prostaglandin E1 treatment. Clin. Exp. Rheumatol. 2012; 30:S44-9.
Buffer:
CCL2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (81%), Horse: (81%), Bovine: (75%), Rabbit: (75%), Guinea pig: (90%)
Immunogen:
Rabbit polyclonal CCL2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human CCL2.

The immunogen is located within the last 50 amino acids of CCL2.
NCBI Gene ID #:
6347
NCBI Official Name:
chemokine (C-C motif) ligand 2
NCBI Official Symbol:
CCL2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 11 kDa

Observed: 14 kDa
Protein Accession #:
NP_002973
Protein GI Number:
4506841
Purification:
CCL2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
Swissprot #:
P13500
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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