NELF Antibody
| Code | Size | Price |
|---|
| PSI-7225-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7225-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
NELF Antibody: HH9, NELF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor
Application Note:
NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Background:
NELF Antibody: NELF (nasal embryonic luteinizing hormone-releasing hormone factor) is a 530 amino acid transcription factor involved in the migration of LHRH neurons, outgrowth of olfactory axons and suppression of transcription elongation. NELF is found in the peripheral and central nervous system during embryonic development, and is highly expressed in adult testis, kidney and brain. Known to couple NMDA receptor signaling to the nucleus, NELF knockdown impaired GnRH neuronal migration of NLT cells in vitro and the gene encoding NELF has been linked to the development of Idiopathic hypogonadotropic hypogonadism (IHH), a disorder resulting in impaired pubertal maturation and reproductive function.
Background References:
- Kramer PR and Wray S. Novel gene expressed in nasal region influences outgrowth of olfactory axons and migration of luteinizing hormone-releasing hormone (LHRH) neurons. Genes Dev. 2000; 14:1824-34.
- Yung TM, Narita T, Komori T, et al. Cellular dynamics of the negative transcription elongation factor NELF. Exp. Cell Res. 2009; 315:1693-705.
- Xu N, Bhagavath B, Kim HG, et al. NELF is a nuclear protein involved in hypothalamic GnRH neuronal migration. Mol. Cell Endocrinol. 2010; 319:47-55.
- Miura K, Acierno JS and Seminara SB. Characterization of the human nasal embryonic LHRH factor gene, NELF, and a mutation screening among 65 patients with idiopathic hypogonadotropic hypogonadism (IHH). J. Hum. Genet. 2004; 49: 265-8.
Buffer:
NELF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal NELF antibody was raised against a 17 amino acid peptide near the center of human NELF.
The immunogen is located within amino acids 230 - 280 of NELF.
The immunogen is located within amino acids 230 - 280 of NELF.
NCBI Gene ID #:
26012
NCBI Official Name:
nasal embryonic LHRH factor
NCBI Official Symbol:
NELF
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 58 kDa
Protein Accession #:
NP_056352
Protein GI Number:
46397396
Purification:
NELF Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
Swissprot #:
Q6X4W1
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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