RC3H1 Antibody
| Code | Size | Price |
|---|
| PSI-7251-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7251-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
RC3H1 Antibody: RNF198, ROQUIN, KIAA2025, RNF198, Roquin-1, RING finger and C3H zinc finger protein 1, Roquin
Application Note:
RC3H1 antibody can be used for detection of RC3H1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
RC3H1 Antibody: The ring finger protein RC3H1, also known as Roquin, is a highly conserved member of the RING type ubiquitin ligase protein family whose M199R mutation leads to the excessive production of follicular helper T cells and germinal centers in the sanroque strain of mice, a strain with excessive IL-21 production and high titers of autoantibodies. The complete loss of RC3H1 induces early death and immune deregulation but not autoimmunity in RC3H1-null mice, suggesting that the mutant RC3H1 is more disruptive to the immune system than its complete loss.
Background References:
- Vinuesa CG, Cook MC, Angelucci C, et al. A RING-type ubiquitin ligase family member required to repress follicular helper T cells and autoimmunity. Nature 2005; 435:452-8.
- Bertossi A, Aichinger M, Sansonetti P, et al. Loss of Roquin induces immune deregulation but not autoimmunity. J. Exp. Med. 2011; 208:1749-56.
Buffer:
RC3H1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal RC3H1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RC3H1.
The immunogen is located within amino acids 1040 - 1090 of RC3H1.
The immunogen is located within amino acids 1040 - 1090 of RC3H1.
NCBI Gene ID #:
149041
NCBI Official Name:
ring finger and CCCH-type domains 1
NCBI Official Symbol:
RC3H1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
125 kDa
Protein Accession #:
NP_742068
Protein GI Number:
73695473
Purification:
RC3H1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
At least three isoforms of RC3H1 are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q5TC82
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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