KREMEN2 Antibody

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ProSci
Product Code: PSI-7263
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7263-0.02mg0.02mg£150.00
Quantity:
PSI-7263-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: KREMEN2, 7263 (2 μg/mL), KREMEN2, 64-235 (1 μg/mL) and beta-actin, 3779 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: KREMEN2, 7263 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Western Blot Validation in Mouse Spleen Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: KREMEN2 7263 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 4 Immunocytochemistry Validation of KREMEN2 in HeLa Cells</strong><br> Immunocytochemical analysis of HeLa cells using anti-KREMEN2 antibody (7263) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: KREMEN2, 7263 (2 μg/mL), KREMEN2, 64-235 (1 μg/mL) and beta-actin, 3779 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: KREMEN2, 7263 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Spleen Tissue Lysate</strong><br> Loading: 15 μg of lysates per lane. Antibodies: KREMEN2 7263 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunocytochemistry Validation of KREMEN2 in HeLa Cells</strong><br> Immunocytochemical analysis of HeLa cells using anti-KREMEN2 antibody (7263) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
KREMEN2 Antibody: KRM2, KRM2, Kremen protein 2, Dickkopf receptor 2
Application Note:
WB: 1-2 μg/mL; ICC: 5 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
KREMEN2 Antibody: Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) co-receptor. Recent experiments have shown that KREMEN2 is a regulator of bone remodeling and raise the possibility that antagonizing KREMEN2 might prove beneficial in patients with bone loss disorders.
Background References:
  • Nakamura?et al. Biochim. Biophys. Acta. 2001; 1518:63-72.
  • Mao et al. Nature 2002; 417:664-7.
  • Li et al. Fertil. Steril. 2008; 90:1470-9.
  • Schulze et al. PLoS 2010; 5:e10309.
Buffer:
KREMEN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Anti-KREMEN2 antibody (7263) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human KREMEN2.

The immunogen is located within amino acids 390-440 of KREMEN2.
ISOFORMS:
Human KREMEN2 has 6 isoforms, including isoform 1 (462aa, 49kD), isoform 2 (424aa, 45kD), isoform 3 (420aa, 44kD), isoform 4 (399aa, 42kD), isoform 5 (423aa, 45kD), and isoform 6 (381aa, 40kD). Mouse KREMEN2 has one isoform (461aa, 49kD) and rat KREMEN2 also has one isoform (468aa, 50kD). 7263 can detect human, mouse and rat.
NCBI Gene ID #:
79412
NCBI Official Name:
kringle containing transmembrane protein 2
NCBI Official Symbol:
KREMEN2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 49kD

Observed: 49kD
Protein Accession #:
NP_757384
Protein GI Number:
27437008
Purification:
KREMEN2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
Multiple isoforms of KREMEN2 are known to exist. KREMEN2 antibody is predicted to not cross-react with other Kremen protein family members.
Swissprot #:
Q8NCW0
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar KREMEN2 expression profile in human, mouse and rat cell lines detected by two independent anti-KREMEN2 antibodies that recognize different epitopes, 7263 against C-terminus domain and 64-235 against central domain. KREMEN2 proteins are detected in most of the tested cell lines at different expression levels by the two independent antibodies.

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