Nucleobindin-2 Antibody
| Code | Size | Price |
|---|
| PSI-7271-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7271-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Nucleobindin-2 Antibody: NEFA, HEL-S-109, NEFA, Nucleobindin-2, DNA-binding protein NEFA
Application Note:
Nucleobindin-2 Antibody can be used for detection of Nucleobindin-2 by Western blot starting at 0.5 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
Nucleobindin-2 Antibody: NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.
Background References:
- Taniguchi N, Taniura H, Niinobe M, et al. The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm. J. Biol. Chem. 2000; 275:31674-85.
- Oh IS, Shimizu H, Satoh T, et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature 2006; 443:709-12.
- Garcia-Galiano D, Navarro VM, Gaytan F, et al. Expanding roles of NUCB2/nesfatin-1 in neuroendocrine regulation. J. Mol. Endocrin. 2010; 45:281-90.
Buffer:
Nucleobindin-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
Nucleobindin-2 antibody was raised against a 16 amino acid peptide near the center of human Nucleobindin-2 .
The immunogen is located within amino acids 260 - 310 of Nucleobindin-2.
The immunogen is located within amino acids 260 - 310 of Nucleobindin-2.
NCBI Gene ID #:
4925
NCBI Official Name:
nucleobindin 2
NCBI Official Symbol:
NUCB2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 46 kDa
Observed: 54 kDa
Observed: 54 kDa
Protein Accession #:
NP_005004
Protein GI Number:
4826870
Purification:
Nucleobindin-2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity
SPECIFICITY:
Nucleobindin-2 antibody is predicted to not cross-react with NUCB1.
Swissprot #:
P80303
User NOte:
Optimal dilutions for each application to be determined by the researcher.
Related Products
| Product Name | Product Code | Supplier | Nucleobindin-2 Peptide | PSI-7271P | ProSci | Summary Details | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|