TIAF Antibody

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ProSci
Product Code: PSI-7305
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7305-0.02mg0.02mg£150.00
Quantity:
PSI-7305-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TIAF in K562 cell lysate with TIAF antibody at 1 μg/mL.
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Immunocytochemistry of TIAF in K562 cells with TIAF antibody at 2.5 μg/ml.
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Western blot analysis of TIAF in K562 cell lysate with TIAF antibody at 1 μg/mL.
Immunocytochemistry of TIAF in K562 cells with TIAF antibody at 2.5 μg/ml.

Documents

Further Information

Additional Names:
TIAF Antibody: MAJN, SPR210, TGFB1-induced anti-apoptotic factor 1, 12 kDa TGF-beta-1-induced antiapoptotic factor
Application Note:
TIAF Antibody can be used for detection of TIAF by Western blot at 1 μg/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
TIAF Antibody: TIAF-1 (TGFB1-induced anti-apoptotic factor 1), also known as MYO18A, is a TGFB1-induced anti-apoptotic factor that functions to inhibit the cytotoxic effects of TNF-alpha, TRADD and FADD. It localizes to the nucleus and significantly up-regulated in activated helper T lymphocytes (TH2) in chronic kidney and liver allograft rejection. Suppression of TIAF1 expression by siRNA prevents UV irradiation-mediated p53 phosphorylation and nuclear translocation. TIAF1 is essential in the p53- and WOX1-mediated cell death and shows an aggregation-dependent control of tumor progression and metastasis, and regulation of cell death.
Background References:
  • Chang NS, Mattison J, Cao H, et al. Cloning and characterization of a novel transforming growth factor-beta1-induced TIAF1 protein that inhibits tumor necrosis factor cytotoxicity. Biochem. Biophys. Res. Commun. 1998; 253:743-9.
  • Khera S and Chang NS. TIAF1 participates in the transforming growth factor beta1--mediated growth regulation. Ann. N.Y. Acad. Sci. 2003; 995:11-21.
  • Van der Leij J, Van den Berg A, Albrecht EW, et al. High expression of TIAF-1 in chronic kidney and liver allograft rejection and in activated T-helper cells. Transplantation 2003; 75:2076-82.
  • Schultz L, Khera S, Sleve D, et al. TIAF1 and p53 functionally interact in mediating apoptosis and silencing of TIAF1 abolishes nuclear translocation of serine 15-phosphorylated p53. DNA Cell Biol. 2004; 23:67-74
Buffer:
TIAF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TIAF antibody was raised against a 16 amino acid peptide near the amino terminus of human TIAF .

The immunogen is located within the first 50 amino acids of TIAF.
NCBI Gene ID #:
9220
NCBI Official Name:
TGFB1-induced anti-apoptotic factor 1
NCBI Official Symbol:
TIAF
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 13 kDa

Observed: 14 kDa
Protein Accession #:
NP_004731
Protein GI Number:
17978509
Purification:
TIAF Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
Two alternatively spliced transcript variants encoding different isoforms have been identified.
Swissprot #:
O95411
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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