ULK1 Antibody
| Code | Size | Price |
|---|
| PSI-7335-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7335-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ULK1 Antibody: ATG1, ATG1A, UNC51, hATG1, Unc51.1, KIAA0722, Autophagy-related protein 1 homolog, ATG1
Application Note:
ULK1 Antibody can be used for detection of ULK1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
ULK1 Antibody: ULK1, also known as ATG1, is a key serine/threonine protein kinase probably acting at the most upstream step of autophagosome formation. Knockout of ULK1 results in a severe defect in the autophagy pathway. ULK1 is highly conserved among eukaryotes, and are the Unc-51-like kinases, ULK1 and ULK2 in mammals. ULK1 is ubiquitously expressed and involved in autophagy in response to starvation. It is the target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13. ULK1 also plays a role early in neuronal differentiation.
Background References:
- Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209?18.
- Lee EJ and Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy 2011; 7:689-95.
- Yan J, Kuroyanagi H, Tomemori T, et al. Mouse ULK2, a novel member of the UNC-51-like protein kinases: unique features of functional domains. Oncogene 1999; 18:5850?9.
- Bach M, Larance M, James DE, et al. The serine/threonine kinase ULK1 is a target of multiple phosphorylation events. Biochem. J. 2011; 440:283-91.
Buffer:
ULK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ULK1 antibody was raised against a 16 amino acid peptide near the center of human ULK1 .
The immunogen is located within amino acids 350 - 400 of ULK1.
The immunogen is located within amino acids 350 - 400 of ULK1.
NCBI Gene ID #:
8408
NCBI Official Name:
unc-51-like kinase 1 (C. elegans)
NCBI Official Symbol:
ULK1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 116 kDa
Observed: 105 kDa
Observed: 105 kDa
Protein Accession #:
NP_003556
Protein GI Number:
4507831
Purification:
ULK1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
Two alternatively spliced transcript variants encoding different isoforms have been identified. ULK1 antibody is predicted to not cross-react with ULK2.
Swissprot #:
O75385
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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