CISD2 Antibody
| Code | Size | Price |
|---|
| PSI-7301-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7301-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
CISD2 Antibody: ERIS, WFS2, ZCD2, NAF-1, Miner1, CDGSH2, ERIS, CDGSH iron-sulfur domain-containing protein 2, Endoplasmic reticulum intermembrane small protein
Application Note:
CISD2 antibody was raised against a 15 amino acid peptide near the center of human CISD2.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
CISD2 Antibody: Defects in the CISD2 (CDGSH iron sulfur domain 2) gene are a cause of the neurodegenerative disorder Wolfram syndrome 2. CISD2 is a zinc finger protein that localizes to the endoplasmic reticulum and mitochondria and binds an iron/sulfur cluster. CISD2 interacts with Bcl-2 and can be displaced by the BH3-only protein BIK and contributes to the regulation of BIK-initiated autophagy. CISD2 deficiency in mice causes mitochondrial breakdown accompanied by autophagic cell death as well as the development of premature aging phenotype.
Background References:
- Amr S, Heisey C, Zhang M, et al. A homozygous mutation in a novel zinc-finger protein, ERIS, is responsible for Wolfram syndrome 2. Am. J. Hum. Genet. 2007; 81:673-83.
- Chang NC, Nguyen M, Germain M, et al. Antagonism of Beclin 1-dependent autophagy by BCL-2 at the endoplasmic reticulum requires NAF-1. EMBO J. 2010; 29:606-18.
- Chen YF, Kao CH, Chen YT, et al. Cisd2 deficiency drives premature aging and causes mitochondria-mediated defects in mice.
Buffer:
CISD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
CISD2 antibody was raised against a 15 amino acid peptide near the center of human CISD2.
The immunogen is located within amino acids 40 - 90 of CISD2.
The immunogen is located within amino acids 40 - 90 of CISD2.
NCBI Gene ID #:
493856
NCBI Official Name:
CDGSH iron sulfur domain 2
NCBI Official Symbol:
CISD2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 15 kDa
Observed: 15 kDa
Observed: 15 kDa
Protein Accession #:
NP_001008389
Protein GI Number:
56605994
Purification:
CISD2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
Swissprot #:
Q8N5K1
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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