APO-E Antibody
| Code | Size | Price |
|---|
| PSI-7333-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7333-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
APO-E Antibody: AD2, LPG, LDLCQ5, Apolipoprotein E, Apo-E
Application Note:
APO-E antibody can be used for detection of APO-E by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
APO-E Antibody: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E (APO-E), a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Defects in APO-E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.
Background References:
- Vasquez EC, Peotta VA, Gava TMC, et al. Cardiac and vascular phenotypes in the epolipoprotein E-deficient mouse. J. Biomed. Sci. 2012; 19:22.
- Feussner G, Funke H, Weng W, et al. Severe type III hyperlipoproteinemia associated with unusual apolipoprotein E1 phenotype and epsilon 1/'null' genotype. Eur. J. Clin. Invest. 1992; 22:599-608.
Buffer:
APO-E Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (78%)
Immunogen:
APO-E antibody was raised against a 19 amino acid peptide near the carboxy terminus of human APO-E.
The immunogen is located within amino acids 220 - 270 of APO-E.
The immunogen is located within amino acids 220 - 270 of APO-E.
NCBI Gene ID #:
348
NCBI Official Name:
apolipoprotein E
NCBI Official Symbol:
APOE
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 35 kDa
Observed: 34 kDa
Observed: 34 kDa
Protein Accession #:
NP_000032
Protein GI Number:
4557325
Purification:
APO-E Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
Swissprot #:
P02649
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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