UNG2 Antibody

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ProSci
Product Code: PSI-7353
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7353-0.02mg0.02mg£150.00
Quantity:
PSI-7353-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of UNG2 in 3T3 cell lysate with UNG2 antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.
2 / 2
Immunofluorescence of UNG2 in 3T3 cells with UNG2 antibody at 20 μg/mL.
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Western blot analysis of UNG2 in 3T3 cell lysate with UNG2 antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.
Immunofluorescence of UNG2 in 3T3 cells with UNG2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
UNG2 Antibody: DGU, UDG, UNG1, UNG2, HIGM4, HIGM5, UNG15, DGU, Uracil-DNA glycosylase
Application Note:
UNG2 antibody can be used for detection of UNG2 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
UNG2 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promoters and alternative splicing. UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from DNA that arise through either misincorporation during replication or cytosine deamination. UNG2 can also be bound by the HIV-1 integrase and incorporated into the virion particle, suggesting that it is required to remove uracils from the viral genome. As the intrinsic antiviral protein APOBEC3G generates numerous uracils in the HIV genome during its replication, it may be that the UNG2 contributes to the APOBEC3G-mediated loss of infectivity by generating abasic sites in the viral genome.
Background References:
  • Krokan HE, Otterlei M, Nilsen H, et al. Properties and functions of human uracil-DNA glycosylase from the UNG gene. Prog. Nucleic Acid Res. Mol. Biol. 2001; 68:365-86.
  • Fromm JC and Verdine GL. Base excision repair. Adv. Protein Chem. 2004; 69:1-41.
  • Willetts KE, Rey F, Agostini I, et al. DNA repair enzyme uracil DNA glycosylase is specifically incorporated into human immunodeficiency virus type 1 viral particles through a Vpr-independent mechanism. J. Virol. 1999; 73:1682-8.
  • Harris RS, Bishop KN, Sheehy AM, et al. DNA deamination mediates innate immunity to retroviral infection. Cell 2003; 113:803-9.
Buffer:
UNG2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
UNG2 antibody was raised against a 17 amino acid peptide near the amino terminus of human UNG2.

The immunogen is located within the first 50 amino acids of UNG2.
NCBI Gene ID #:
7374
NCBI Official Name:
uracil-DNA glycosylase
NCBI Official Symbol:
UNG
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 34 kDa

Observed: 33 kDa
Protein Accession #:
NP_550433
Protein GI Number:
19718751
Purification:
UNG2 Antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
SPECIFICITY:
At least two isoforms of UNG2 are known to exist; this antibody will only detect the longer isoform. UNG2 antibody is predicted to not cross-react with UNG1.
Swissprot #:
P13051
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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