TLX3 Antibody

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ProSci
Product Code: PSI-7375
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7375-0.02mg0.02mg£150.00
Quantity:
PSI-7375-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of TLX3 in human spleen tissue lysate with TLX3 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of TLX3 in human spleen tissue with TLX3 antibody at 2.5 μg/ml.
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Immunofluorescence of TLX3 in human spleen tissue with TLX3 antibody at 20 μg/ml.
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Western blot analysis of TLX3 in human spleen tissue lysate with TLX3 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of TLX3 in human spleen tissue with TLX3 antibody at 2.5 μg/ml.
Immunofluorescence of TLX3 in human spleen tissue with TLX3 antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
TLX3 Antibody: RNX, HOX11L2, T-cell leukemia homeobox protein 3, Homeobox protein Hox-11L2
Application Note:
TLX3 antibody can be used for detection of TLX3 by Western blot at 0.5 - 1 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TLX3 Antibody: The Hox proteins play a role in patterns of embryonic development and cellular differentiation by regulating downstream target genes. TLX3, also known as homeobox11-like1 (HOX11L2), is a DNA-binding nuclear transcription factor that is expressed in a subset of the primary sensory nervous system. TLX3, along with the related TLX1, is a selector transcription factor that promotes an excitatory glutamatergic neuronal phenotype over an inhibitory GABAergic phenotype opposing LBX1 signals during dorsal spinal cord development. Chromosomal translocations of the TLX3 gene have been shown to result in some forms of T-cell acute lymphoblastic leukemia (T-ALL).
Background References:
  • Dear TN, Sanchez-Garcia I, and Rabbitts TH, et al. The Hox11 gene encodes a DNA-binding nuclear transcription factor belonging to a distinct family of homeobox genes. Proc. Natl. Acad. Sci. USA 1993; 90:4431-5.
  • Patterson KD and Kried PA. Hox11-family genes XHox11 and XHox11L2 in xenopus: XHoxL2 expression is restricted to a subset of the primary sensory neurons. Dev. Dyn. 1999; 214:34-43.
  • Cheng L, Arata A, Mizuguchi R, et al. Tlx3 and Tlx1 are post-mitotic selector genes determining the glutamatergic over GABAergic cell fates. Nat. Neurosci. 2004; 7:510-7.
  • Cheng L, Samad OA, Xu Y, et al. Lbx1 and Tlx3 are opposing switches in determining GABAergic versus glutamatergic transmitter phenotypes. Nat. Neurosci. 2005; 8:1510-5.
Buffer:
TLX3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
TLX3 antibody was raised against a 16 amino acid peptide near the amino terminus of human TLX3.

The immunogen is located within the first 50 amino acids of TLX3.
NCBI Gene ID #:
30012
NCBI Official Name:
T-cell leukemia homeobox 3
NCBI Official Symbol:
TLX3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 32 kDa

Observed: 30 kDa
Protein Accession #:
NP_066305
Protein GI Number:
50355978
Purification:
TLX3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
TLX3 antibody is predicted to not cross-react with other TLX proteins.
Swissprot #:
O43711
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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