HIV-1 p24 Monoclonal Antibody

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ProSci
Product Code: PSI-PM-6585
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-PM-6585-0.02mg0.02mg£150.00
Quantity:
PSI-PM-6585-0.1mg0.1mg£515.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 p24 antibody PM-6585 at (A) 0.5 μg/mL and (B) 0.2 μg/mL, respectively.
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Titration ELISA of anti-HIV-1 p24 antibody PM-6585 with 100 ng of recombinant HIV-1 p24 protein.
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Sandwich ELISA analysis of recombinant p24 proteins using capture antibody [7F4] and biotin labeled detection antibody [8G9]. (Assay sensitivity = 2.5 pg of recombinant p24 protein).
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Western blot analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 p24 antibody PM-6585 at (A) 0.5 μg/mL and (B) 0.2 μg/mL, respectively.
Titration ELISA of anti-HIV-1 p24 antibody PM-6585 with 100 ng of recombinant HIV-1 p24 protein.
Sandwich ELISA analysis of recombinant p24 proteins using capture antibody [7F4] and biotin labeled detection antibody [8G9]. (Assay sensitivity = 2.5 pg of recombinant p24 protein).

Documents

Further Information

Additional Names:
HIV-1 p24 Antibody [7F4] :
Application Note:
PM-6585 can be used for detection of p24 by Western blot or ELISA at 0.2 ? 0.5μg/mL.
Background:
HIV-1 p24 Monoclonal Antibody: The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core and the region between the two termed matrix (1). The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein (2). The p24 constitutes the major core component of the virus and shows high degree of sequence conservation among HIV isolates. The Gag p24 has been used as an integral part of multicomponent HIV-1 vaccines (3).
Background References:
  • Goto T, Nakai M, and Ikuta K. The life-cycle of human immunodeficiency virus type 1. Micron 1998; 29:123-38.
  • Freed EO. HIV-1 gag proteins: diverse functions in the virus life cycle. Virology 1998; 251:1-15.
  • Flynn BJ, Kastenmuller K, Wille-Reece U, et al. Immunization with HIV Gag targeted to dendritic cells followed by recombinant New York vaccinia virus induces robust T-cell immunity in nonhuman primates. Proc. Natl. Acad. Sci. USA 2011; 108:7131?6.
Buffer:
HIV-1 p24 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Mouse monoclonal HIV-1 p24 antibody was raised against a recombinant full-length HIV-1 p24 protein.
NCBI Gene ID #:
155030
NCBI Official Name:
gag Pr55(Gag)
NCBI Official Symbol:
GAG
NCBI Organism:
hiv-1
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 24 kDa, 41 kDa, and 55 kDa
Protein Accession #:
AAB50258
Protein GI Number:
327745
Purification:
HIV-1 p24 antibody is supplied as protein A purified IgG1.
Research Area:
Infectious Disease,Antibody Pairs
SPECIFICITY:
By Western blot, anti-HIV-1 p24 antibody detects a ~24 kDa, a ~41 kDa, and a ~55 kDa protein, corresponding to HIV-1 p24 and to its precursors p41 and p55, respectively, in HIV-1 samples.
Swissprot #:
P04591
User NOte:
Optimal dilutions for each application to be determined by the researcher.