SCUBE3 Antibody

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ProSci
Product Code: PSI-5847
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-5847-0.02mg0.02mg£150.00
Quantity:
PSI-5847-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)

Images

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Immunohistochemistry of SCUBE3 in human blood vessel tissue with SCUBE3 antibody at 10 μg/mL.
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Immunohistochemistry of SCUBE3 in human blood vessel tissue with SCUBE3 antibody at 10 μg/mL.

Documents

Further Information

Additional Names:
SCUBE3 Antibody: CEGF3, CEGF3, Signal peptide, CUB and EGF-like domain-containing protein 3
Application Note:
SCUBE3 antibody can be used for detection of SCUBE3 by immunohistochemistry at 5 μg/mL.

Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
Background:
SCUBE3 Antibody: SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart (1,2). Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth. SCUBE3 has been shown to be an endogenous TGF-beta receptor ligand (3,4) and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-beta receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-beta1, VEGF, Snail, and Slug.
Background References:
  • Wu BT, Su YH, Tsai MT, et al. A novel secreted, cell-surface glycoprotein containing multiple epidermal growth factor-like repeats and one CUB domain is highly expressed in primary osteoblasts and bones. J. Biol. Chem. 2004; 279:37485-90.
  • Yang RB, Ng CK, Wasserman SM, et al. Identification of a novel family of cell-surface proteins expressed in human vascular endothelium. J. Biol. Chem. 2002; 277:46364-73.
  • Yang HY, Cheng CF, Djoko B, et al. Transgenic overexpression of the secreted, extracellular EGF-CUB domain containing protein SCUBE3 induces cardiac hypertrophy in mice. Cardiovas. Res. 2007; 75:139-47.
  • Wu YY, Peck K, Chang YL, et al. SCUBE3 is an endogenous TGF- receptor ligand and regulates the epithelial-mesenchymal transition in lung cancer. Oncogene 2011; 30:3682-93.
Buffer:
SCUBE3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
SCUBE3 antibody was raised against a 15 amino acid peptide near the center of human SCUBE3.

The immunogen is located within amino acids 440 - 490 of SCUBE3.
NCBI Gene ID #:
222663
NCBI Official Name:
signal peptide, CUB and EGF-like domain-containing protein 3 precursor
NCBI Official Symbol:
SCUBE3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 109 kDa
Protein Accession #:
NP_689966
Protein GI Number:
31377568
Purification:
SCUBE3 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
Swissprot #:
Q8IX30
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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