GABARAP Antibody
| Code | Size | Price |
|---|
| PSI-7359-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7359-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
GABARAP Antibody: MM46, ATG8A, GABARAP-a, FLC3B, HT004, Gamma-aminobutyric acid receptor-associated protein, MM46
Application Note:
GABARAP antibody can be used for detection of GABARAP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
GABARAP Antibody: Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system. It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. GABARAP (GABAA receptor-associated protein) links GABAA receptors to the cytoskeleton and may play a role in intracellular transport of GABAA receptors and its interaction with the cytoskeleton. GABARAP belongs to the MAP1 or ATG8 like family and recent studies show that MAPK15/ERK8 is acting through interaction with ATG8 family proteins to regulate autophagy.
Background References:
- Cherubini E, Gaiarsa JL, and Ben-Ari Y. GABA: an excitatory transmitter in early postnatal life. Trends Neurosci.1991; 14:515-19.
- Dirkx R Jr, Thomas A, Li L, et al. Targeting of the 67 kDa isoform of glutamic acid decarboxylase to intracellular organelles is mediated by its interaction with the NH2-terminal region of the 65 kDa isoform of glutamic acid decarboxylase. J. Biol. Chem. 1995; 270:2241-6.
- Wang H, Bedford FK, Brandon NJ, et al. GABA(A)-receptor-associated protein links GABAA receptors and the cytoskeleton. Nature 1999; 397:69-72.
- Colecchia D, Strambi A, Sanzone S, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 2012 Sep 4;8(12).
Buffer:
GABARAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%)
Immunogen:
GABARAP antibody was raised against a 19 amino acid peptide near the amino terminus of human GABARAP.
The immunogen is located within the first 50 amino acids of GABARAP.
The immunogen is located within the first 50 amino acids of GABARAP.
NCBI Gene ID #:
11337
NCBI Official Name:
GABA(A) receptor-associated protein
NCBI Official Symbol:
GABARAP
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 13kDa
Observed: 15 kDa
Observed: 15 kDa
Protein Accession #:
NP_009209
Protein GI Number:
6005764
Purification:
GABARAP Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
Multiple isoforms of GABARAP exist due to alternative splicing events.
Swissprot #:
O95166
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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