N-RAS Antibody
| Code | Size | Price |
|---|
| PSI-7381-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7381-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
N-RAS Antibody: NS6, ALPS4, N-ras, NRAS1, HRAS1, GTPase NRas, Transforming protein N-Ras
Application Note:
N-RAS antibody can be used for detection of N-RAS by Western blot at 1 and 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Background:
N-RAS Antibody: Activating mutations and overexpression of classical Ras subfamily members (N-RAS, H-RAS, and K-RAS) have been widely investigated as key events in the development of human cancers. The N-RAS protein shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. N-RAS, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
Background References:
- Ehrhardt A, Ehrhardt GR, Guo X, et al. Ras and relatives--job sharing and networking keep an old family together. Exp. Hematol. 2002; 30:1089-106.
- Swarthout JT, Lobo S, Farh L, et al. DHHC9 and GCP9 constitute a human protein fatty acyltransferase with specificity for H- and N-Ras. J. Biol. Chem. 2005; 280:31141-8.
- Malaney S and Daly RJ. The ras signaling pathway in mammary tumorigenesis and metastasis. J. Mammary Gland Biol. Neoplasia 2001; 6:101-13.
- Rodenhuis S. ras and human tumors. Semin Cancer Biol. 1992; 3:241-7
Buffer:
N-RAS Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Guinea pig: (93%), Chicken: (80%)
Immunogen:
N-RAS antibody was raised against a 15 amino acid peptide near the carboxy terminus of human N-RAS.
The immunogen is located within the last 50 amino acids of N-RAS.
The immunogen is located within the last 50 amino acids of N-RAS.
NCBI Gene ID #:
4893
NCBI Official Name:
neuroblastoma RAS viral (v-ras) oncogene homolog
NCBI Official Symbol:
NRAS
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 22 kDa
Observed: 22 kDa
Observed: 22 kDa
Protein Accession #:
NP_002515
Protein GI Number:
4505451
Purification:
N-RAS Antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
N-RAS antibody is predicted to not cross-react with H-RAS
Swissprot #:
P01111
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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