GOLGA5 Antibody
| Code | Size | Price |
|---|
| PSI-7387-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7387-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
GOLGA5 Antibody: RFG5, GOLIM5, ret-II, RETII, RFG5, PIG31, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein
Application Note:
GOLGA5 antibody can be used for detection of GOLGA5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
GOLGA5 Antibody: GOLGA5 (Golgi autoantigen, subfamily A, member 5), also known as GOLGA84, RFG5 (RET-fused gene 5 protein) or GOLIM5, is a ubiquitously expressed single-pass type II coiled-coil membrane protein that is involved in maintaining Golgi structure (1). It is localized to the Golgi apparatus and predominantly found on membranes at the cis side of the Golgi stack (1,2). As a mitotic phosphoprotein, GOLGA5 binds to active Rab 1 and associates with CASP (an isoform of CDP) in a golgin-tethering complex that is believed to play a role in intra-Golgi retrograde transport (3,4). Defects in GOLGA5 are a cause of thyroid papillary carcinoma (TPC) (5).
Background References:
- Bascom RA, Srinivasan S, Nussbaum RL. Identification and characterization of golgin 84, a novel Golgi integral membrane protein with a cytoplasmic coiled-coil domain. J. Biol. Chem. 1999; 274:2953-62.
- Diao A, Rahman D, Pappin DJ, et al. 2003. The coiled-coil membrane protein golgin 84 is a novel Rab effector required for Golgi ribbon formation. J. Cell Biol. 2003; 160:201-12.
- Malsam J, Satoh A, Pelletier L, et al. Golgin tethers define subpopulations of COPI vesicles. Science 2005; 307:1095-8.
- Sohda M, Misumi Y, Yamamoto A, et al. Interaction of Golgin-84 with the COG complex mediates the intra-Golgi retrograde transport. Traffic 2010;11:1552-66.
Buffer:
GOLGA5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GOLGA5 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human GOLGA5.
The immunogen is located within amino acids 540 - 590 of GOLGA5.
The immunogen is located within amino acids 540 - 590 of GOLGA5.
NCBI Gene ID #:
9950
NCBI Official Name:
golgin A5
NCBI Official Symbol:
GOLGA5
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 64, 80 kDa
Observed: 65, 78 kDa
Observed: 65, 78 kDa
Protein Accession #:
NP_005104
Protein GI Number:
30260188
Purification:
GOLGA5 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
GOLGA5 antibody is human, mouse and rat reactive. Two isoforms of GOLGA5 exist due to alternative splicing events.
Swissprot #:
Q8TBA6
User NOte:
Optimal dilutions for each application to be determined by the researcher.
Related Products
| Product Name | Product Code | Supplier | GOLGA5 Peptide | PSI-7387P | ProSci | Summary Details | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|