DAP Antibody
| Code | Size | Price |
|---|
| PSI-7389-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7389-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
DAP Antibody: DAP1, Death-associated protein 1, DAP-1
Application Note:
DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).
Background References:
- Deiss LP, Feinstein E, Berissi H, et al. Identification of a novel serine/threonine kinase and a novel 15-kD protein as potential mediators of the gamma interferon-induced cell death. Genes Dev. 1995; 9:15-30.
- Koren I, Reem E, and Kimchi A. DAP1, a novel substrate of mTOR, negatively regulates autophagy. Curr. Biol. 2010; 20:1093-8.
Buffer:
DAP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (89%)
Immunogen:
DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP.
The immunogen is located within the last 50 amino acids of DAP.
The immunogen is located within the last 50 amino acids of DAP.
NCBI Gene ID #:
1611
NCBI Official Name:
death-associated protein
NCBI Official Symbol:
DAP
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 11 kDa
Observed: 11 kDa
Observed: 11 kDa
Protein Accession #:
NP_004385
Protein GI Number:
4758120
Purification:
DAP Antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
DAP antibody is human, mouse and rat reactive.
Swissprot #:
P51397
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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