LPIN1 Antibody

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ProSci
Product Code: PSI-7393
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7393-0.02mg0.02mg£150.00
Quantity:
PSI-7393-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of LPIN1 in K562 cell lysate with LPIN1 antibody at 1 μg/mL.
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Immunohistochemistry of LPIN1 in human small intestine tissue with LPIN1 antibody at 5 μg/ml.
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Immunofluorescence of LPIN1 in human small intestine tissue with LPIN1 antibody at 20 μg/ml.
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Western blot analysis of LPIN1 in K562 cell lysate with LPIN1 antibody at 1 μg/mL.
Immunohistochemistry of LPIN1 in human small intestine tissue with LPIN1 antibody at 5 μg/ml.
Immunofluorescence of LPIN1 in human small intestine tissue with LPIN1 antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
LPIN1 Antibody: PAP1, KIAA0188, Phosphatidate phosphatase LPIN1, Lipin-1
Application Note:
LPIN1 antibody can be used for detection of LPIN1 by Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
LPIN1 Antibody: LPIN1, also known as PAP1, is a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol (reviewed in 1). LPIN1 is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism (1,2). Mutations in LPIN1 are associated with metabolic diseases such as type 2 diabetes and recurrent acute myoglobinuria (3,4) and it is also a candidate for several human lipodystrophy syndromes (5).
Background References:
  • Reue K and Zhang P. The lipin protein family: dual roles in lipid biosynthesis and gene expression. FEBS Lett. 2008; 582:90-6.
  • Peterfy M, Phan J, Xu P, et al. Lipodystrophy in the fld mouse results from mutation of a new gene encoding a nuclear protein, lipin. Nat. Genet. 2001; 27:121-4.
  • Zhang R, Jiang F, Hu C, et al. Genetic variants of LPIN1 indicate an association with Type 2 diabetes mellitus in a Chinese population. Diabet. Med. 2013; 30:118-22.
  • Zeharia A, Shaag A, Houtkooper RH, et al. Mutations in LPIN1 cause recurrent acute myoglobinuria in childhood. Am. J. Hum. Genet. 2008; 83:489-94.
Buffer:
LPIN Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
LPIN1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human LPIN1.

The immunogen is located within the last 50 amino acids of LPIN1.
Molecular Weight Explanation:
Other LPIN1 antibodies show similar size band
NCBI Gene ID #:
23175
NCBI Official Name:
lipin 1
NCBI Official Symbol:
LPIN1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 107 kDa

Observed: 125 kDa
Protein Accession #:
NP_001248357
Protein GI Number:
387528013
Purification:
LPIN1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
LPIN1 antibody is human and mouse reactive. At least four isoforms of LPIN1 are known to exist.
Swissprot #:
Q14693
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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