NCBP1 Antibody
| Code | Size | Price |
|---|
| PSI-7395-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7395-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
NCBP1 Antibody: NCBP, Sto1, CBP80, NCBP, Nuclear cap-binding protein subunit 1, 80 kDa nuclear cap-binding protein
Application Note:
NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Background:
NCBP1 Antibody: NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1,2). Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3).
Background References:
- Ohno M, Kataoka N, and Shimura Y. A nuclear cap binding protein from HeLa cells. Nuc. Acid Res. 1990; 18:6989-95.
- Maquat LE, Hwang J, Sato H, et al. CBP80-promoted mRNP rearrangements during the pioneer round of translation, nonsense-mediated mRNA decay, and thereafter. Cold Spring Harbor Symp. Quant. Biol. 2010; 75:127-34.
- Bier K, York A, and Fodor E. Cellular cap-binding proteins associate with influenza virus mRNAs. J. Gen. Virol. 92:1627-34.
Buffer:
NCBP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (81%)
Immunogen:
NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1.
The immunogen is located within amino acids 420 - 470 of NCBP1.
The immunogen is located within amino acids 420 - 470 of NCBP1.
NCBI Gene ID #:
4686
NCBI Official Name:
nuclear cap binding protein subunit 1, 80kDa
NCBI Official Symbol:
NCBP1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 87 kDa
Observed: 87 kDa
Observed: 87 kDa
Protein Accession #:
NP_002477
Protein GI Number:
4505343
Purification:
NCBP1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist; this antibody will detect both isoforms of NCBP1.
Swissprot #:
Q09161
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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