Bim EL Antibody
| Code | Size | Price |
|---|
| PSI-7421-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7421-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Bim EL Antibody: BAM, BIM, BOD, Bcl-2-like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11
Application Note:
Bim EL antibody can be used for detection of Bim EL by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Bim EL Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim, also known as Bcl-2-like protein 11, is a pro-apoptotic member of this family and interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Multiple isoforms of Bim are known to exist, with Bim EL being the longest isoform (1,2).
Background References:
- O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
- Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.
Buffer:
Bim EL Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Bim EL antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bim.
The immunogen is located within amino acids 20 - 70 of Bim EL.
The immunogen is located within amino acids 20 - 70 of Bim EL.
NCBI Gene ID #:
10018
NCBI Official Name:
BCL2-like 11 (apoptosis facilitator)
NCBI Official Symbol:
BCL2L11
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 22 kDa
Observed: 24 kDa
Observed: 24 kDa
Protein Accession #:
NP_619527
Protein GI Number:
20336315
Purification:
Bim EL Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
Bim EL antibody is human and mouse reactive. Multiple isoforms of Bim are known to exist; this antibody only detects the Bim EL isoform.
Swissprot #:
O43521
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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