ESR1 Antibody
| Code | Size | Price |
|---|
| PSI-7437-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7437-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ESR1 Antibody: ER, ESR, Era, ESRA, ESTRR, NR3A1, Estrogen receptor, ER-alpha, ER
Application Note:
ESR1 antibody can be used for detection of ESR1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Background:
ESR1 Antibody: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).
Background References:
- Pakdel F, Reese JC, and Katzenellenbogen BS. Identification of charged residues in an N- terminal portion of the hormone-binding domain of the human estrogen receptor important in transcriptional activity of the receptor. Mol. Endocrinol. 1993; 7:1408-17.
- Sheeler CQ, Singleton DW, and Khan SA. Mutation of serines 104, 106, and 118 inhibits dimerization of the human estrogen receptor in yeast. Endocr. Res. 2003; 29:237-55.
- Rogatsky I, Trowbridge JM, and Garabedian MJ. Potentiation of human estrogen receptor alpha transcriptional activation through phosphorylation of serines 104 and 106 by the cyclin A-CDK2 complex. J. Biol. Chem. 1999; 274:22296-302.
Buffer:
ESR1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%), Horse: (100%)
Immunogen:
ESR1 antibody was raised against an 18 amino acid peptide near the center of human ESR1.
The immunogen is located within amino acids 250 - 300 of ESR1.
The immunogen is located within amino acids 250 - 300 of ESR1.
NCBI Gene ID #:
2099
NCBI Official Name:
estrogen receptor 1
NCBI Official Symbol:
ESR1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 65 kDa
Observed: 62 kDa
Observed: 62 kDa
Protein Accession #:
NP_001116214
Protein GI Number:
170295804
Purification:
ESR1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
ESR1 antibody is human, mouse and rat reactive.
Swissprot #:
P03372
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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