DDC Antibody
| Code | Size | Price |
|---|
| PSI-7447-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7447-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
DDC Antibody : AADC, AADC, Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase
Application Note:
DDC antibody can be used for detection of DDC by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
DOPA decarboxylase (DDC) belongs to the group II decarboxylase family of proteins (1). It is an important protein in the catecholamine biosynthesis pathway. DDC catalyzes the second reaction in the biosynthesis of catecholamines, trace amines and serotonin (1,2). It can form a homodimer and is expressed in the central nervous system (2). DDC can be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease, neuroendocrine tumors and drug abuse (3,4). Defects in DDC gene may cause the autosomal recessive disorder AADC deficiency (4).
Background References:
- Berry MD, Juorio AV, Li XM, et al. Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem. Res. 1996; 21:1075-87.
- Sumi-Ichinose C, Ichinose H, Takahashi E, et al. Molecular cloning of genomic DNA and chromosomal assignment of the gene for human aromatic L-amino acid decarboxylase, the enzyme for catecholamine and Serotonin biosynthesis. Biochemistry 1992; 31:2229-38.
- Haycock JW, Becker L, Ang L, et al. Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J. Neurochem. 2003; 87:574-85.
- Chang YT, Sharma R, Marsh JL, et al. Levodopa-responsive aromatic L-amino acid decarboxylase deficiency. Ann. Neurol. 2004; 55:435-8.
Buffer:
DDC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (92%), Guinea pig: (77%), Pig: (77%)
Immunogen:
DDC antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DDC.
The immunogen is located within amino acids 390 - 440 of DDC .
The immunogen is located within amino acids 390 - 440 of DDC .
NCBI Gene ID #:
1644
NCBI Official Name:
dopa decarboxylase (aromatic L-amino acid decarboxylase)
NCBI Official Symbol:
DDC
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 53 kDa
Observed: 53 kDa
Observed: 53 kDa
Protein Accession #:
NP_000781
Protein GI Number:
4503281
Purification:
DDC Antibody is affinity chromatography purified via peptide column.
Research Area:
Neuroscience
SPECIFICITY:
DDC antibody is human, mouse and rat reactive.
Swissprot #:
P20711
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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