FLI1 Antibody

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ProSci
Product Code: PSI-7449
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7449-0.02mg0.02mg£150.00
Quantity:
PSI-7449-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Western Blot (WB)

Images

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Western blot analysis of FLI1 in Jurkat cell lysate with FLI1 antibody at (A) 0.5 and (B) 1 μg/ml.
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Immunofluorescence of FLI1 in HeLa cells with MUC1 antibody at 5 μg/mL.
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Western blot analysis of FLI1 in Jurkat cell lysate with FLI1 antibody at (A) 0.5 and (B) 1 μg/ml.
Immunofluorescence of FLI1 in HeLa cells with MUC1 antibody at 5 μg/mL.

Documents

Further Information

Additional Names:
FLI1 Antibody: EWSR2, SIC-1, BDPLT21
Application Note:
FLI1 antibody can be used for detection of FLI1 by Western blot at 0.5 - 1 μg/ml.

Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The Friend leukemia virus integration 1 (FLI1) protein is a transcription factor containing an ETS DNA-binding domain and is highly expressed in hematopoietic cell lineages and vascular endothelial cells (1). FLI1 plays important roles in megakaryocytic differentiation (2) and vascular homeostasis (3). The FLI1 gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. (4). Abnormal expression of FLI1 can be used as an adverse prognostic indicator for acute myeloid leukemia (5).
Background References:
  • Hollenhorst PC, Jones DA, and Graves BJ. Expression profiles frame the promoter specificity dilemma of the ETS family of transcription factors. Nuc. Acids Res. 2004; 32:5693-702.
  • Spyropoulos DD, Pharr PN, Lavenburg KR, et al. Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor. Mol. Cell Biol. 2000; 20:5643-52.
  • Asano Y, Stawski L, Hant F, et al. Endothelial Fli1 deficiency impairs vascular homeostasis: a role in scleroderma vasculopathy. Am. J. Pathol. 2010; 176:1983-98.
  • May WA, Gishizky ML, Lessnick SL, et al. Ewing sarcoma 11;22 translocation produces a chimeric transcription factor that requires the DNA-binding domain encoded by FLI1 for transformation. Proc. Natl. Acad. Sci. USA 1993; 90:5752-6. 5. Kornblau SM, Qiu YH, Zhang N, et al. Abnormal expression of FLI1 protein is an adverse prognostic factor in acute myeloid leukemia. Blood 2011; 118:5604-12.
Buffer:
FLI1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
FLI1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human FLI1.

The immunogen is located within the last 50 amino acids of FLI1 .
NCBI Gene ID #:
2313
NCBI Official Name:
Fli-1 proto-oncogene, ETS transcription factor
NCBI Official Symbol:
FLI1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 50 kDa

Observed: 52kDa
Protein Accession #:
NP_002008
Protein GI Number:
7110593
Purification:
FLI1 Antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
FLI1 antibody is human, mouse and rat reactive. At least four isoforms of FLI1 are known to exist; this antibody will detect all four.
Swissprot #:
Q01543
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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