FHIT Antibody
| Code | Size | Price |
|---|
| PSI-7455-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7455-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Western Blot (WB)
Documents
Further Information
Additional Names:
FHIT Antibody : FRA3B, AP3AaseAP3A hydrolase, AP3Aase
Application Note:
FHIT antibody can be used for detection of FHIT by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
FHIT is member of the histidine triad gene family and is a diadenosine involved in purine metabolism (1). FHIT is also thought to be a tumor suppressor gene and is involved in multiple apoptotic pathways (1,2). The FHIT gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene (3). Aberrant transcripts from this gene have been found in multiple carcinomas (4).
Background References:
- Barnes LD, Garrison PN, Siprashvili Z, et al. Fhit, a putative tumor suppressor in humans, is a dinucleotide 5',5'''-P1,P3-triphosphate hydrolase. Biochemistry 1996; 35:11529-35.
- Wali A. FHIT: doubts are clear now. ScientificWorldJournal 2010; 10:1142-51.
- Ohta M, Inoue H, Cotticelli MG, et al. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. Cell 1996; 84:587-97.
- Drusco A, Pekarsky Y, Costinean S, et al. Common fragile site tumor suppressor genes and corresponding mouse models of cancer. J. Biomed. Biotechnol.2011; Epub 2010 Dec 29.
Buffer:
FHIT Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%), Rat: (72%)
Immunogen:
FHIT antibody was raised against an 18 amino acid peptide near the carboxy terminus of human FHIT.
The immunogen is located within the last 50 amino acids of FHIT .
The immunogen is located within the last 50 amino acids of FHIT .
NCBI Gene ID #:
2272
NCBI Official Name:
fragile histidine triad
NCBI Official Symbol:
FHIT
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 16 kDa
Observed: 15kDa
Observed: 15kDa
Protein Accession #:
NP_002003
Protein GI Number:
4503719
Purification:
FHIT Antibody is affinity chromatography purified via peptide column.
Research Area:
Apoptosis
SPECIFICITY:
FHIT antibody is human and mouse reactive.
Swissprot #:
P49789
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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