ARSB Antibody

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ProSci
Product Code: PSI-7465
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7465-0.02mg0.02mg£150.00
Quantity:
PSI-7465-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of ARSB in mouse lung tissue lysate with ARSB antibody at (A) 1 and (B) 2 μg/ml.
2 / 3
Immunohistochemistry of ARSB in human lung tissue with ARSB antibody at 2.5 μg/mL.
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Immunofluorescence of ARSB in human lung tissue with ARSB antibody at 20 μg/mL.
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Western blot analysis of ARSB in mouse lung tissue lysate with ARSB antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of ARSB in human lung tissue with ARSB antibody at 2.5 μg/mL.
Immunofluorescence of ARSB in human lung tissue with ARSB antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
ARSB Antibody : ASB, G4S, MPS6, Arylsulfatase B, N-acetylgalactosamine-4-sulfatase, ASB
Application Note:
ARSB antibody can be used for detection of ARSB by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Aryl sulfatase B (ARSB) forms a homodimer that hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate (1). ARSB localizes to the lysozyme as well as to the extracellular matrix (2). Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B (3), and can be treated with exogenous ARSB (4).
Background References:
  • Wasserman SI and Austen KF. Arylsulfatase B of human lung. Isolation, characterization, and interaction with slow-reacting substance of anaphylaxis. J. Clin. Invest. 1976; 57:738-44.
  • Mitsunaga-Nakatsubo K, Kusunoki S, Kawakami H, et al. Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian livers. Med. Mol. Morphol. 2009; 42:63-9.
  • Valayannopoulos V, Nicely H, Harmatz P, et al. Mucopolysaccharidosis VI. Orphanet. J. Rare Dis. 2010; 5:5.
  • Harmatz P, Whitley CB, Waber L, et al. Enzyme replacement therapy in mucopolysaccharidosis VI (Maroteaux-Lamy syndrome). J. Pediatr. 2004; 144:574-80.
Buffer:
ARSB Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ARSB antibody was raised against a 16 amino acid peptide near the carboxy terminus of human ARSB.

The immunogen is located within amino acids 460 - 510 of ARSB .
NCBI Gene ID #:
411
NCBI Official Name:
arylsulfatase B
NCBI Official Symbol:
ARSB
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 59 kDa

Observed: 57kDa
Protein Accession #:
NP_000037
Protein GI Number:
38569405
Purification:
ARSB Antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
ARSB antibody is human, mouse and rat reactive. At least two isoforms of ARSB are known to exist; this antibody only recognizes the longest isoform.
Swissprot #:
P15848
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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