MMP9 Antibody
| Code | Size | Price |
|---|
| PSI-7471-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7471-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Documents
Further Information
Additional Names:
MMP9 Antibody : GELB, CLG4B, MMP-9, MANDP2, Matrix metalloproteinase-9, 92 kDa gelatinase
Application Note:
MMP9 antibody can be used for detection of MMP9 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The matrix metalloproteinase (MMP) family are a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases (1). MMP9 degrades type IV and V collagens (2) and studies suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow (3) and plays a role in tumor-associated tissue remodeling (4).
Background References:
- Iyer RP, Patterson NL, Fields GB, et al. The history of matrix metalloproteinases: milestones, myths, and misperceptions. Am. J. Heart Circ. Physiol 2012; 303:H919-30.
- Murphy G, Knauper V, Atkinson S, et al. Matrix metalloproteinases in arthritic disease. Arthritis Res. 2002; 4 Suppl. 3:S39-49.
- Sweeney EA, Lortat-Jacob H, Priestley GV, et al. Sulfated polysaccharides increase plasma levels of SDF-1 in monkeys and mice: involvement in mobilization of stem/progenitor cells. Blood 2002; 99:44-51.
- Rhee JW, Lee KW, Sohn WJ, et al. Regulation of matrix metalloproteinase-9 gene expression and cell migration by NF-kappa B in response to CpG-oligodeoxynucleotides in RAW 264.7 cells. Mol. Immunol. 2007; 44:1393-400.
Buffer:
MMP9 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%)
Immunogen:
MMP9 antibody was raised against a 16 amino acid peptide near the center of human MMP9.
The immunogen is located within amino acids 260 - 310 of MMP9 .
The immunogen is located within amino acids 260 - 310 of MMP9 .
NCBI Gene ID #:
4318
NCBI Official Name:
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
NCBI Official Symbol:
MMP9
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 76 kDa
Observed: 73kDa
Observed: 73kDa
Protein Accession #:
NP_004985
Protein GI Number:
74272287
Purification:
MMP9 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
MMP9 antibody is human, mouse and rat reactive. At least three isoforms of MMP9 are known to exist; this antibody only recognizes the two longest isoforms.
Swissprot #:
P14780
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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