IL-37 Antibody

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ProSci
Product Code: PSI-7499
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7499-0.02mg0.02mg£150.00
Quantity:
PSI-7499-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)

Images

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Western blot analysis of IL-37 in human lung tissue lysate with IL-37 antibody at 1 μg/ml in (A) the presence and (B) the absence of blocking peptide.
2 / 3
Immunohistochemistry of IL-12A in human kidney tissue with IL-12A antibody at 5 μg/mL.
3 / 3
Immunofluorescence of IL-12A in human kidney tissue with IL-12A antibody at 20 μg/mL.
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Western blot analysis of IL-37 in human lung tissue lysate with IL-37 antibody at 1 μg/ml in (A) the presence and (B) the absence of blocking peptide.
Immunohistochemistry of IL-12A in human kidney tissue with IL-12A antibody at 5 μg/mL.
Immunofluorescence of IL-12A in human kidney tissue with IL-12A antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
IL-37 Antibody : FIL1, FIL1Z, IL-1H, IL-37, IL1F7, IL1H4, IL-1F7, IL-1H4, IL1RP1, IL-1RP1, FIL1(ZETA), Interleukin-37, FIL1 zeta
Application Note:
IL-37 antibody can be used for detection of IL-37 by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Interleukin 37 (IL-37) is a member of the interleukin 1 cytokine family (reviewed in 1). It can bind to, and may be a ligand for interleukin 18 receptor (IL18R1/IL-1Rrp). IL-37 also binds to interleukin 18 binding protein (IL18BP), an inhibitory binding protein of interleukin 18 (IL18), and subsequently forms a complex with IL18 receptor beta subunit, and through which it inhibits the activity of IL18 (1). IL-37 can also interact with Smad3 and expression of IL-37 in macrophages or epithelial cells resulted in near complete suppression of pro-inflammatory cytokines, suggesting that IL-37 may act as a natural suppressor of the innate inflammatory and immune responses (2,3).
Background References:
  • Boraschi D, Lucchesi D, Hainzl S, et al. IL-37: a new anti-inflammatory cytokine of the IL-1 family. Eur. Cytokine Netw. 2011; 22:127-47.
  • Nold MF, Nold-Petry CA, Zepp JA, et al. Interleukin 37 is a fundamental inhibitor of innate immunity. Nat. Immunol. 2010; 11:1014-22.
  • Sakai N, Van Sweringen HL, Belizaire RM, et al. Interleukin-37 reduces liver inflammatory injury via effects on hepatocytes and non-parenchymal cells. J. Gastroenterol. Hepatol. 2012; 27:1609-16.
Buffer:
IL-37 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
IL-37 antibody was raised against a 17 amino acid peptide near the amino terminus of human IL-37.

The immunogen is located within amino acids 30 - 80 of IL-37 .
NCBI Gene ID #:
27178
NCBI Official Name:
interleukin 37
NCBI Official Symbol:
IL37
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 24 kDa

Observed: 23kDa
Protein Accession #:
NP_055254
Protein GI Number:
20127524
Purification:
IL-37 Antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
IL-37 antibody is human specific. Multiple isoforms of IL-37 are known to exist; this antibody will only detect the largest isoform. IL-37 antibody is predicted to not cross-react with IL-36.
Swissprot #:
Q9NZH6
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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