TFEB Antibody

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ProSci
Product Code: PSI-6803
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-6803-0.02mg0.02mg£150.00
Quantity:
PSI-6803-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)

Images

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Immunohistochemistry of TFEB in human lung tissue with TFEB antibody at 2.5 μg/mL.
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Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 μg/mL.
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Immunohistochemistry of TFEB in human lung tissue with TFEB antibody at 2.5 μg/mL.
Immunofluorescence of TFEB in human lung tissue with TFEB antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
TFEB Antibody: TCFEB, BHLHE35, ALPHATFEB, Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35
Application Note:
TFEB antibody can be used for detection of TFEB by immunohistochemistry at 2.5 μg/ml.

Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The Transcription factor EB (TFEB) is a member in the basic helix-loop-helix leucine zipper superfamily of transcription factors that is translocated in a subset of renal tumors (1,2). Recent studies have shown that lysosomal biogenesis is regulated by TFEB (3), which is in turn regulated by the mammalian target of rapamycin (mTOR) complex 1 (4). Other evidence suggests that TFEB coordinates the major steps of the autophagic pathway by driving the expression of autophagy and lysosomal genes (5).
Background References:
  • Carr CS and Sharp PA. A helix-loop-helix protein related to the immunoglobulin E box-binding proteins. Mol. Cell Biol. 1990; 10:4384-8.
  • Davis IJ, Hsi BL, Arroyo JD, et al. Cloning of an alpha-TFEB fusion in renal tumors harboring the t(6;11)(p21;q13) chromosome translocation. Proc. Natl. Acad. Sci. USA 2003; 100:6051-6.
  • Sardiello M, Palmieri M, di Ronza A, et al. A gene network regulating lysosomal biogenesis and function. Science 2009; 325:473-7.
  • Pena-Llopis S, Vega-Rubin-de-Celis S, Schwartz JC, et al. Regulation of TFEB and V-ATPases my mTORC1. EMBO J. 2011; 30:3242-58.
Buffer:
TFEB antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Rabbit polyclonal TFEB antibody was raised against a 16 amino acid peptide near the carboxy terminus of human TFEB.

The immunogen is located within amino acids 390 - 440 of TFEB.
NCBI Gene ID #:
7942
NCBI Official Name:
transcription factor EB
NCBI Official Symbol:
TFEB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
N/A
Protein Accession #:
NP_009093
Protein GI Number:
24307933
Purification:
TFEB antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
TFEB antibody is human specific. At least three isoforms of TFEB are known to exist.
Swissprot #:
P19484
User NOte:
Optimal dilutions for each application to be determined by the researcher.