DPF3 Antibody

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ProSci
Product Code: PSI-7559
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7559-0.02mg0.02mg£150.00
Quantity:
PSI-7559-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of DPF3 in mouse brain tissue lysate with DPF3 antibody at (A) 1 and (B) 2 μg/ml.
2 / 3
Immunohistochemistry of DPF3 in human brain tissue with DPF3 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of DPF3 in human brain tissue with DPF3 antibody at 20 μg/mL.
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Western blot analysis of DPF3 in mouse brain tissue lysate with DPF3 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of DPF3 in human brain tissue with DPF3 antibody at 5 μg/mL.
Immunofluorescence of DPF3 in human brain tissue with DPF3 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
DPF3 Antibody: CERD4, BAF45C, CERD4, Zinc finger protein DPF3, BRG1-associated factor 45C
Application Note:
DPF3 antibody can be used for detection of DPF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The DPF3 protein, also known as Cerd4, is a member of the d4 gene family of transcription modulators that also includes DPF1/Neud4 and DPF2/Requiem (1). DPF3 has been shown to be a epigenetic key factor for heart and muscle development and can bind to methylated and acetylated lysine residues of histone 3 and 4, suggesting that DPF3 may play a role in recruiting chromatin remodeling complexes to acetylated histones (2). Two isoforms of DPF3, DPF3a and DPF3b, are required as transcriptional co-activators in SWI/SNF complex-dependent activation of the NF-kappaB RelA/p50 heterodimer (3).
Background References:
  • Ninkina NN, Mertsalov IB, Kulikova DA, et al. Cerd4, third member of the d4 gene family: expression and organization of genomic locus. Mamm. Genome 2001; 12:862-6.
  • Lange M, Kaynak B, Forster UB. Regulation of muscle development by DBF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex. Genes Dev. 2008; 22:2370-84.
  • Ishizaka A, Mizutani T, Kobayashi K, et al. Double plant homeodomain (PHD) finger proteins DPF3a and -3b are required as transcriptional co-activators in SWI/SNF complex-dependent activation of NF-kappaB RelA/p50 heterodimer. J. Biol. Chem. 2012; 287:11924-33.
Buffer:
DPF3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (83%)
Immunogen:
DPF3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DPF3.

The immunogen is located within amino acids 220 - 270 of DPF3.
NCBI Gene ID #:
8110
NCBI Official Name:
D4, zinc and double PHD fingers, family 3
NCBI Official Symbol:
DPF3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 39 kDa

Observed: 50 kDa
Protein Accession #:
NP_036206
Protein GI Number:
148762958
Purification:
DPF3 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist.
Swissprot #:
Q92784
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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