ULK3 Antibody
| Code | Size | Price |
|---|
| PSI-7585-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7585-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ULK3 Antibody: Unc-51-like kinase 3
Application Note:
ULK3 antibody can be used for detection of ULK3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1). Knockout of ULK genes results in a severe defect in the autophagy pathway (2). ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3). ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4). Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins; this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5).
Background References:
- Suzuki K, Kubota Y, Sekito T, et al. Hierarchy of Atg proteins in pre-autophagosomal structure organization. Genes to Cells 2007; 12:209?18.
- Lee EJ and Tournier C. The requirement of uncoordinated 51-like kinase 1 (ULK1) and ULK2 in the regulation of autophagy. Autophagy 2011; 7:689-95.
- Zhou X, Babu JR, da Silva S, et al. Unc-51-like kinase 1/2-mediated endocytic processes regulate filopodia extension and branching of sensory axons. Proc. Natl. Acad. Sci. USA 2007; 104:5842-7.
- Maloverjan A, Piirsoo M, Michelson P, et al. Identification of a novel serine/threonine kinase ULK3 as a positive regulator of Hedgehog pathway. Exp. Cell Res. 2010; 316:627-37.
Buffer:
ULK3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (95%), Mouse: (89%), Chicken: (79%)
Immunogen:
ULK3 antibody was raised against a 19 amino acid peptide near the center of human ULK3.
The immunogen is located within amino acids 230 - 280 of ULK3.
The immunogen is located within amino acids 230 - 280 of ULK3.
NCBI Gene ID #:
25989
NCBI Official Name:
unc-51 like kinase 3
NCBI Official Symbol:
ULK3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 52 kDa
Observed: 52 kDa
Observed: 52 kDa
Protein Accession #:
NP_001092906
Protein GI Number:
150456432
Purification:
ULK3 antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.
Swissprot #:
Q6PHR2
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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