Alpha-tubulin Antibody
| Code | Size | Price |
|---|
| PSI-7599-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7599-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
Alpha-tubulin Antibody: TUBA2, bA408E5.3, TUBA2, TUBA3D, Alpha-tubulin 2
Application Note:
Alpha-tubulin antibody can be used for detection of alpha-tubulin by Western blot at 0.25 - 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
Background References:
- McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.
- Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53.
- Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.
Buffer:
Alpha-tubulin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Pig: (100%), Chicken: (100%)
Immunogen:
Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin.
The immunogen is located within the first 50 amino acids of Alpha-tubulin.
The immunogen is located within the first 50 amino acids of Alpha-tubulin.
NCBI Gene ID #:
7278
NCBI Official Name:
tubulin, alpha 3c
NCBI Official Symbol:
TUBA3C
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 50 kDa
Observed: 45 kDa
Observed: 45 kDa
Protein Accession #:
NP_005992
Protein GI Number:
17921993
Purification:
Alpha-tubulin antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
Alpha-tubulin antibody is human, mouse and rat reactive.
Swissprot #:
Q13748
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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