TESPA1 Antibody

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ProSci
Product Code: PSI-7601
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7601-0.02mg0.02mg£150.00
Quantity:
PSI-7601-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of TESPA1 in A431 cell lysate with TESPA1 antibody at 1 μg/ml.
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Immunocytochemistry of TESPA1 in A431 cells with TESPA1 antibody at 5 μg/mL.
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Immunofluorescence of TESPA1 in A431 cells with TESPA1 antibody at 20 μg/mL.
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Western blot analysis of TESPA1 in A431 cell lysate with TESPA1 antibody at 1 μg/ml.
Immunocytochemistry of TESPA1 in A431 cells with TESPA1 antibody at 5 μg/mL.
Immunofluorescence of TESPA1 in A431 cells with TESPA1 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
TESPA1 Antibody: HSPC257, KIAA0748, HSPC257, Protein TESPA1, Thymocyte-expressed positive selection-associated protein 1
Application Note:
TESPA1 antibody can be used for detection of TESPA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TESPA1 is a critical component of the TCR signalosome and is essential for T cell selection and maturation through the regulation of TCR signaling during T cell development (1). TESPA1 encodes a protein of 458 amino acids and is highly conserved among vertebrate species (2). TESPA1 expression was tightly regulated during T cell development, with highest expression at the DP stage (3). TESPA1 deficiency resulted in failure of positive selection and substantially fewer of CD4+ and CD8+ SP cells in the thymus (1,4).
Background References:
  • Wang D, Zheng M, Lei L, et al. Tespa1 is involved in late thymocyte development through the regulation of TCR-mediated signaling. Nat. Immunol. 2012; 13:560-8.
  • Matsuzaki H, Fujimoto T, Ota T, et al. Tespa1 is a novel inositol 1,4,5-trisphosphate receptor binding protein in T and B lymphocytes. FEBS Open Bio. 2012; 2:255-9.
  • Matsuzaki H, Fujimoto T, Tanaka M, et al. Tespa1 is a novel component of mitochondria-associated endoplasmic reticulum membranes and affects mitochondrial calcium flux. Biochem. Biophys. Res. Commun. 2013; 433:322-6.
  • Fujimoto T, Matsuzaki H, Tanaka M, et al. Tespa1 protein is phosphorylated in response to store-operated calcium entry. Biochem. Biophys. Res. Commun. 2013; 434:162-5.
Buffer:
TESPA1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen:
TESPA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human TESPA1.

The immunogen is located within amino acids 20 - 70 of TESPA1.
NCBI Gene ID #:
9840
NCBI Official Name:
thymocyte expressed, positive selection associated 1
NCBI Official Symbol:
TESPA1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 57 kDa

Observed: 60 kDa
Protein Accession #:
NP_001129502
Protein GI Number:
209862861
Purification:
TESPA1 antibody is affinity chromatography purified via peptide column.
Research Area:
Immunology
SPECIFICITY:
TESPA1 antibody is human specific. At least three isoforms TESPA1 are known to exist.
Swissprot #:
A2RU30
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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