PHC3 Antibody
| Code | Size | Price |
|---|
| PSI-7607-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7607-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PHC3 Antibody: EDR3, HPH3, EDR3, PH3, Polyhomeotic-like protein 3, Early development regulatory protein 3, hPH3
Application Note:
PHC3 antibody can be used for detection of PHC3 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development (1). PHC3 (Polyhomeotic-like protein 3), also known as Early development regulatory protein 3, is a 983 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex (2). PRC1 complex acts via chromatin remodeling and modification of histones. PHC3 contains 1 FCS-type zinc finger and SAM (sterile alpha motif) domain (2-3). PHC 3 has been regarded as a candidate tumor suppressor of osteosarcoma (4).
Background References:
- Valk-Lingbeek ME, Bruggeman SW, and Van Lohuizen M. Stem cells and cancer; the polycomb connection. Cell 2004; 118:409?18.
- Deshpande AM, Akunowicz JD, Reveles XT, et al. PHC3, a component of the hPRC-H complex, associates with E2F6 during G0 and is lost in osteosarcoma tumors. Oncogene 2007; 26:1714-22.
- Isono K, Fujimura Y, Shinga J, et al. Mammalian polyhomeotic homologues Phc2 and Phc1 act in synergy to mediate polycomb repression of Hox genes. Mol. Cell Biol. 2005; 25:6694-706.
- Iwata S, Takenobu H, Kageyama H, et al. Polycomb group molecule PHC3 regulates polycomb complex composition and prognosis of osteosarcoma. Cancer Sci. 2010; 101:1646-52.
Buffer:
PHC3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PHC3 antibody was raised against a 19 amino acid peptide near the amino terminus of human PHC3.
The immunogen is located within amino acids 240 - 290 of PHC3.
The immunogen is located within amino acids 240 - 290 of PHC3.
NCBI Gene ID #:
80012
NCBI Official Name:
polyhomeotic homolog 3 (Drosophila)
NCBI Official Symbol:
PHC3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 109 kDa
Observed: 120 kDa
Observed: 120 kDa
Protein Accession #:
NP_079223
Protein GI Number:
148612879
Purification:
PHC3 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
PHC3 antibody is human, mouse and rat specific. At least six isoforms of PHC3 are known to exist.
Swissprot #:
Q8NDX5
User NOte:
Optimal dilutions for each application to be determined by the researcher.
Related Products
| Product Name | Product Code | Supplier | PHC3 Peptide | PSI-7607P | ProSci | Summary Details | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|