AQP2 Antibody
| Code | Size | Price |
|---|
| PSI-7621-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7621-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
AQP2 Antibody: AQP-CD, WCH-CD, Aquaporin-2, ADH water channel, AQP-2
Application Note:
AQP2 antibody can be used for detection of AQP2 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
Aquaporins are membrane proteins that serve in the transfer of water and small solutes across cellular membranes. One such aquaporin, aquaporin-2 (AQP2) is located in the kidney collecting tubule and plays a critical role in water reabsorbtion (1). AQP2 is mainly localized in intracellular vesicles but upon stimulation with anti-diuretic hormone (ADH), AQP2 is translocated to the apical plasma membrane by exocytic fusion of AQP2-bearing vesicles (2). Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus (3).
Background References:
- Denker BM, Smith BL, Kuhada FP, et al. Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J. Biol. Chem. 1988; 263:15634-42.
- Barile M, Pisitkun T, Yu MJ, et al. Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting duct. Mol. Cell Proteomics 2005; 4:1095-106.
- Bockenhauer D and Bichet DG. Inherited secondary nephrogenic diabetes insipidus: concentrating on humans. Am. J. Physiol. Renal Physiol. 2013; 304:F1037-42.
Buffer:
AQP2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (100%), Bovine: (95%)
Immunogen:
AQP2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AQP2.
The immunogen is located within the last 50 amino acids of AQP2.
The immunogen is located within the last 50 amino acids of AQP2.
NCBI Gene ID #:
359
NCBI Official Name:
aquaporin 2 (collecting duct)
NCBI Official Symbol:
AQP2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 30 kDa
Observed: 28 kDa
Observed: 28 kDa
Protein Accession #:
NP_000477
Protein GI Number:
4502179
Purification:
AQP2 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
AQP2 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with other members of the aquaporin protein family.
Swissprot #:
P41181
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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