GRK6 Antibody
| Code | Size | Price |
|---|
| PSI-7641-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7641-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
GRK6 Antibody: GPRK6, GPRK6, G protein-coupled receptor kinase 6, G protein-coupled receptor kinase GRK6
Application Note:
GRK6 antibody can be used for detection of GRK6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The G protein?coupled receptor kinases (GRKs) are a versatile family of kinases that play a critical role in cancer metastasis through their regulation of G-protein coupled receptors (GPCRs) involved in growth factor mediated cell migration (1,2). Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state (1). GRK6 is one of 7 members of the GRK serine/threonine kinase subfamily, which has been shown to modulate the Wnt signaling pathway via phosphorylation of LRP6 (3,4), and the insulin-like growth factor signaling pathway (4). GRK6 may also play a role in immune system function (5).
Background References:
- Inglese J, Freedman NJ, Koch WJ, et al. Structure and mechanism of the G protein-coupled receptor kinases. J. Biol. Chem. 1993; 268:23735-8.
- Raghuwanshi SK, Smith N, Rivers EJ, et al. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J. Immunol. 2013; 190:5329-36.
- Benovic JL and Gomez J. Molecular cloning and expression of GRK6. A new member of the G protein-coupled receptor kinase family. J. Biol. Chem. 1993; 268:19521-7.
- Chen M, Philipp M, Wang J, et al. G Protein-coupled receptor kinases phosphorylate LRP6 in the Wnt pathway. J. Biol. Chem. 2009; 284:35040-8.
Buffer:
GRK6 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
GRK6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human GRK6.
The immunogen is located within the last 50 amino acids of GRK6.
The immunogen is located within the last 50 amino acids of GRK6.
NCBI Gene ID #:
2870
NCBI Official Name:
G protein-coupled receptor kinase 6
NCBI Official Symbol:
GRK6
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 63 kDa
Observed: 63 kDa
Observed: 63 kDa
Protein Accession #:
NP_001004106
Protein GI Number:
51896039
Purification:
GRK6 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer,Cell Cycle
SPECIFICITY:
GRK6 antibody is human, mouse and rat reactive. Multiple isoforms of GRK6 are known to exist. This antibody is predicted to not cross-react with other members of the GRK protein family.
Swissprot #:
P43250
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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