ARR3 Antibody
| Code | Size | Price |
|---|
| PSI-7643-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7643-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ARR3 Antibody: ARRX, ARRX, CAR, Arrestin-C, Cone arrestin, C-arrestin
Application Note:
ARR3 antibody can be used for detection of ARR3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Background:
Arrestin 3 (ARR3) belongs to the Arrestin family of proteins that function as negative regulators of G protein-coupled receptor (GPCR) signaling (1,2). ARR3 binds the c-Jun N-terminal kinase 3 (JNK3) and scaffolds the apoptosis signal-regulating kinase 1 (ASK1)-MAPK kinase4-JNK3 cascade in a receptor-independent fashion, promoting JNK3 phosphorylation (3,4). ARR3 also mediates the internalization of the C-C chemokine receptor CCR7 upon the binding of its ligand CCL19, but not CCL21, suggesting that it helps mediate CCR7 receptor desensitization (5).
Background References:
- Murakami A, Yajima T, Sakuma H, et al. X-arrestin: a new retinal arrestin mapping to the X chromosome. FEBS Lett. 1993; 334:203-9.
- Gurevich VV and Gurevich EV. The structural basis of arrestin-mediated regulation of G-protein-coupled receptors. Pharmacol. Ther. 2006; 110:465-502.
- Song X, Coffa S, Fu H, et al. How does arrestin assemble MAPKs into a signaling complex J. Biol. Chem. 2009; 284:685-95.
- Seo J, Tsakem EL, Breitman M, et al. Identification of arrestin-3-specific residues necessary for JNK3 kinase activation. J. Biol. Chem. 2011; 286:27894-901.
Buffer:
ARR3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ARR3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ARR3.
The immunogen is located within the last 50 amino acids of ARR3.
The immunogen is located within the last 50 amino acids of ARR3.
NCBI Gene ID #:
407
NCBI Official Name:
arrestin 3, retinal (X-arrestin)
NCBI Official Symbol:
ARR3
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 43 kDa
Observed: 47 kDa
Observed: 47 kDa
Protein Accession #:
NP_004303
Protein GI Number:
156071467
Purification:
ARR3 antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
ARR3 antibody is human, mouse and rat reactive. At least three isoforms of ARR3 are known to exist. This antibody is predicted to not cross-react with other members of the arrestin protein family.
Swissprot #:
P36575
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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