ITCH Antibody

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ProSci
Product Code: PSI-7645
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7645-0.02mg0.02mg£150.00
Quantity:
PSI-7645-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of ITCH in 3T3 cell lysate with ITCH antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Western blot analysis of ITCH in 3T3 cell lysate with ITCH antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.

Documents

Further Information

Additional Names:
ITCH Antibody: AIF4, AIP4, NAPP1, dJ468O1.1, E3 ubiquitin-protein ligase Itchy homolog, Atrophin-1-interacting protein 4, Itch
Application Note:
ITCH antibody can be used for detection of ITCH by Western blot at 1 - 2 μg/ml.

Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Background:
The Itchy E3 ubiquitin protein ligase (ITCH) is a member of the Nedd4 family of HECT domain E3 ubiquitin ligases (1). HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. ITCH plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses (2). In B cells, ITCH is thought to associate with latent membrane protein 2A (LMP2A) of Epstein-Barr virus, specifically down-regulating its activity in B cell signaling (3). Mutations in this gene are a cause of syndromic multisystem autoimmune disease (4).
Background References:
  • Perry WL, Hustad CM, Swing DA, et al. The itchy locus encodes a novel ubiquitin protein ligase that is disrupted in a18H mice. Nat. Genet. 1998; 18:143-6.
  • Melino G, Gallagher E, Aqeilan RI, et al. Itch: a HECT-type E3 ligase regulating immunity, skin and cancer. Cell Death Differ. 2008; 15:1103-12.
  • Ikeda A, Caldwell RG, Longnecker R, et al. Itchy, a Nedd4 ubiquitin ligase, downregulates latent membrane protein 2A activity in B-cell signaling. J. Virol. 2003; 77:5529-34.
  • Matesic LE, Copeland NG, and Jenkins NA. Itchy mice: the identification of a new pathway for the development of autoimmunity. Curr. Top. Microbiol. Immunol. 2008; 321:185-200.
Buffer:
ITCH antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ITCH antibody was raised against a 15 amino acid peptide near the amino terminus of human ITCH .

The immunogen is located within amino acids 40 - 90 of ITCH.
NCBI Gene ID #:
83737
NCBI Official Name:
itchy E3 ubiquitin protein ligase
NCBI Official Symbol:
ITCH
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 99 kDa

Observed: 99 kDa
Protein Accession #:
NP_001244066
Protein GI Number:
380420335
Purification:
ITCH antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
ITCH antibody is human, mouse and rat reactive. At least three isoforms of ITCH are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.
Swissprot #:
Q96J02
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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