SPINSTER Antibody

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ProSci
Product Code: PSI-7651
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7651-0.02mg0.02mg£150.00
Quantity:
PSI-7651-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of SPINSTER in HepG2 cell lysate with SPINSTER antibody at (A) 1 and (B) 2 μg/ml.
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Immunohistochemistry of SPINSTER in human lung tissue with SPINSTER antibody at 2.5 μg/ml.
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Immunofluorescence of SPINSTER in human lung tissue with SPINSTER antibody at 20 μg/ml.
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Western blot analysis of SPINSTER in HepG2 cell lysate with SPINSTER antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of SPINSTER in human lung tissue with SPINSTER antibody at 2.5 μg/ml.
Immunofluorescence of SPINSTER in human lung tissue with SPINSTER antibody at 20 μg/ml.

Documents

Further Information

Additional Names:
SPINSTER Antibody: LAT, nrs, SPIN1, SPINL, HSpin1, PP2030, PP20300, Protein spinster homolog 1
Application Note:
SPINSTER antibody can be used for detection of SPINSTER by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
SPINSTER, also known as SPNS1 or SPIN1, is a 528 amino acid multi-pass membrane protein that localizes to the inner mitochondrial membrane and belongs to the spinster subfamily of the major facilitator superfamily (1). SPINSTER interacts with Bcl-x and Bcl-2 and, via this interaction, is thought to be involved in necrotic or autophagic cell death (2). The related protein SPNS2 is critical for the normal lymphocyte localization and mammalian immune system function (1,3).
Background References:
  • Saier MH Jr, Beatty JT, Goffeau A, et al. The major facilitator superfamily. J. Mol. Microbiol. Biotechnol. 1999; 1:257-79.
  • Yanagisawa H, Miyashita T, Nakano Y, et al. HSpin1, a transmembrane protein interacting with Bcl-2/Bcl-xL, induces a caspase-independent autophagic cell death. Cell Death Differ. 2003; 10:798-807.
  • Nakano Y, Fujitani K, Kurihara J, et al. Mutations in the novel membrane protein spinster interfere with programmed cell death and cause neural degeneration in Drosophila melanogaster. Mol. Cell. Biol. 2001; 21:3775-88.
Buffer:
SPINSTER antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (83%), Rat: (81%), Mouse: (81%)
Immunogen:
SPINSTER antibody was raised against an 18 amino acid peptide near the amino terminus of human SPINSTER.

The immunogen is located within the first 50 amino acids of SPINSTER.
NCBI Gene ID #:
83985
NCBI Official Name:
spinster homolog 1 (Drosophila)
NCBI Official Symbol:
SPNS1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 58 kDa

Observed: 56 kDa
Protein Accession #:
NP_001135920
Protein GI Number:
215490098
Purification:
SPINSTER antibody is affinity chromatography purified via peptide column.
Research Area:
Autophagy
SPECIFICITY:
SPINSTER antibody is human specific. At least four isoforms of SPINSTER are known to exist. This antibody is predicted to not cross-react with other members of the spinster family of proteins.
Swissprot #:
Q9H2V7
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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