ARF6 Antibody
| Code | Size | Price |
|---|
| PSI-7699-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7699-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ARF6 Antibody: ADP-ribosylation factor 6
Application Note:
ARF6 antibody can be used for detection of ARF6 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The ADP-ribosylation factor 6 (ARF6) is a member of the ARF family, which is part of the RAS superfamily (1). The ARF proteins are small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D (2). The product of this gene is localized to the plasma membrane (3), and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling (4).
Background References:
- Graves RA, Tontonoz P, and Spiegelman BM. Analysis of a tissue-specific enhancer: ARF6 regulates adipogenic gene expression. Mol. Cell Biol. 1992; 12:1202-8.
- Moss J and Vaughan M. Activation of toxin ADP-ribosyltransferases by eukaryotic ADP-ribosylation factors. Mol Cell Biochem. 1999; 193:153-7.
- Cavenagh MM, Whitney JA, Carroll K, et al. Intracellular distribution of Arf proteins in mammalian cells. Arf6 is uniquely localized to the plasma membrane. J. Biol. Chem. 1996; 271:21767-74.
- Schweitzer JK, Sedgewick AE, and D'Souza-Schorey C. ARF6-mediated endocytic recycling impacts cell movement, cell division and lipid homestasis. Semin. Cell Dev. Biol. 2011; 22:39-47.
Buffer:
ARF6 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (100%)
Immunogen:
ARF6 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ARF6.
The immunogen is located within the last 50 amino acids of ARF6.
The immunogen is located within the last 50 amino acids of ARF6.
NCBI Gene ID #:
382
NCBI Official Name:
ADP-ribosylation factor 6
NCBI Official Symbol:
ARF6
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 19 kDa
Observed: 18 kDa
Observed: 18 kDa
Protein Accession #:
NP_001654
Protein GI Number:
4502211
Purification:
ARF6 antibody is affinity chromatography purified via peptide column.
Research Area:
Signal Transduction
SPECIFICITY:
ARF6 antibody is human, mouse and rat reactive.
Swissprot #:
P62330
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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