SUZ12 Antibody

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ProSci
Product Code: PSI-7701
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7701-0.02mg0.02mg£150.00
Quantity:
PSI-7701-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of SUZ12 in human liver tissue lysate with SUZ12 antibody at 1 μg/ml.
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Immunohistochemistry of SUZ12 in human lung tissue with SUZ12 antibody at 5 μg/mL.
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Immunofluorescence of SUZ12 in human lung tissue with SUZ12 antibody at 20 μg/mL.
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Western blot analysis of SUZ12 in human liver tissue lysate with SUZ12 antibody at 1 μg/ml.
Immunohistochemistry of SUZ12 in human lung tissue with SUZ12 antibody at 5 μg/mL.
Immunofluorescence of SUZ12 in human lung tissue with SUZ12 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
SUZ12 Antibody: CHET9, JJAZ1, CHET9, KIAA0160, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, ChET 9 protein
Application Note:
SUZ12 antibody can be used for detection of SUZ12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis (1). SUZ12 (suppressor of zeste 12) is a 90 - 95 kDa member of the polycomb group of transcriptional regulators (2). It is part of a transcription repression complex termed PRC2 (Polycomb repressive complex 2) that methylates histones in the nucleus, resulting in homeotic (pattern-inducing) gene silencing. It contains one C2H2-type zinc finger region and a C-terminal VEFS-box (3,4). SUZ12 is overexpressed in breast and colon cancer. SUZ12 may be a cause of endometrial stromal tumors (5).
Background References:
  • Kirmizis A, Bartley SM, Kuzmichev A, et al. Silencing of human polycomb target genes is associated with methylation of Histone H3 Lys 27. Genes Dev. 2004; 18:1592-605.
  • Pasini D, Bracken AP, Hansen JB, et al. The polycomb group protein Suz12 is required for embryonic stem cell differentiation. Mol. Cell. Biol. 2007; 27:3769-79.
  • Cao R and Zhang Y. SUZ12 is required for both the Histone methyltransferase activity and the silencing function of the EED-EZH2 complex. Mol. Cell 2004; 15:57-67.
  • Pasini D, Bracken AP, Jensen MR, et al. SUZ12 is essential for mouse development and for EZH2 Histone methyltransferase activity. EMBO J. 2004; 23:4061-71.
Buffer:
SUZ12 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (79%)
Immunogen:
SUZ12 antibody was raised against a 19 amino acid peptide near the center of human SUZ12.

The immunogen is located within amino acids 400 - 450 of SUZ12.
NCBI Gene ID #:
23512
NCBI Official Name:
SUZ12 polycomb repressive complex 2 subunit
NCBI Official Symbol:
SUZ12
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 81 kDa

Observed: 96 kDa
Protein Accession #:
NP_056170
Protein GI Number:
197333809
Purification:
SUZ12 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
SUZ12 antibody is human specific.
Swissprot #:
Q15022
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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