PPARGC1A Antibody
| Code | Size | Price |
|---|
| PSI-7705-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7705-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
PPARGC1A Antibody: LEM6, PGC1, PGC1A, PGC-1v, PPARGC1, PGC-1(alpha), LEM6, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Ligand effect modulator 6, PGC-1-alpha
Application Note:
PPARGC1A antibody can be used for detection of PPARGC1A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), also known as LEM-6, is a transcriptional coactivator that regulates the genes involved in energy metabolism (1). PPARGC1A interacts with PPARgamma, which permits the interaction of PPARGC1A with multiple transcription factors. PPARGC1A can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination (2). PPARGC1A may be also involved in the development of obesity (3).
Background References:
- Tsuemi T and La Spada AR. PGC-1a at the intersection of bioenergetics regulation and neuron function: from Huntington's disease to Parkinson's disease and beyond. Prog. Neurobiol. 2012; 97:142-51.
- Kang C and Li Ji L. Role of PGC-1a signaling in skeletal muscle health and disease. Ann. NY Acad. Sci. 2012; 1271:110-7.
- Liu C and Lin JD. PGC-1 coactivators in the control of energy metabolism. Acta Biochim. Biophys. Sin. 2011; 43:248-57.
Buffer:
PPARGC1A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (100%)
Immunogen:
PPARGC1A antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PPARGC1A.
The immunogen is located within the last 50 amino acids of PPARGC1A.
The immunogen is located within the last 50 amino acids of PPARGC1A.
NCBI Gene ID #:
10891
NCBI Official Name:
peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
NCBI Official Symbol:
PPARGC1A
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 90 kDa
Observed: 88 kDa
Observed: 88 kDa
Protein Accession #:
NP_037393
Protein GI Number:
7019499
Purification:
PPARGC1A antibody is affinity chromatography purified via peptide column.
Research Area:
Obesity
SPECIFICITY:
PPARGC1A antibody is human, mouse and rat reactive. At least three isoforms of PPARGC1A are known to exist; this antibody only detects the longest isoform.
Swissprot #:
Q9UBK2
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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