ME1 Antibody
| Code | Size | Price |
|---|
| PSI-7721-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7721-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
ME1 Antibody: MES, HUMNDME, NADP-dependent malic enzyme, Malic enzyme 1, NADP-ME
Application Note:
ME1 antibody can be used for detection of ME1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Background:
ME1, also known as NADP-ME, MES or HUMNDME, may play an important role as a housekeeping protein within the cell (1). ME1 is a 572 amino acid cytoplasmic protein that belongs to the malic enzyme family (2). It is expressed ubiquitously with highest expression in liver and white adipose tissue. ME1 functions as an NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (3). The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles (3,4). ME1 is regulated by both thyroid hormone levels and the amount of carbohydrates in the diet (5).
Background References:
- Loeber G, Dworkin MB, Infante A, et al. Characterization of cytosolic malic enzyme in human tumor cells. FEBS Lett. 1994; 344:181-6.
- Gonzalez-Manchon C, Ferrer M, Ayuso MS, et al. Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver. Gene 1995;159:255-60.
- Gonzalez-Manchon C, Butta N, Ferrer M, et al. Molecular cloning and functional characterization of the human cytosolic malic enzyme promoter: thyroid hormone responsiveness. DNA Cell Biol. 1997;16:533-44.
- Yang Z, Lanks CW and Tong L. Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate. Structure 2002; 10:951-60.
Buffer:
ME1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ME1 antibody was raised against a 16 amino acid peptide near the amino terminus of human ME1.
The immunogen is located within the first 50 amino acids of ME1.
The immunogen is located within the first 50 amino acids of ME1.
NCBI Gene ID #:
4199
NCBI Official Name:
malic enzyme 1, NADP(+)-dependent, cytosolic
NCBI Official Symbol:
ME1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 63 kDa
Observed: 63 kDa
Observed: 63 kDa
Protein Accession #:
NP_002386
Protein GI Number:
4505143
Purification:
ME1 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
ME1 antibody is human, mouse and rat reactive. ME1 antibody is predicted not to cross-react with ME2.
Swissprot #:
P48163
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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