ME2 Antibody

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ProSci
Product Code: PSI-7723
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7723-0.02mg0.02mg£150.00
Quantity:
PSI-7723-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2μg/ml.
2 / 3
Immunohistochemistry of ME2 in human spleen tissue with ME2 antibody at 5 μg/mL.
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Immunofluorescence of ME2 in human spleen tissue with ME2 antibody at 20 μg/mL.
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Western blot analysis of ME2 in human spleen tissue lysate with ME2 antibody at (A) 1 and (B) 2μg/ml.
Immunohistochemistry of ME2 in human spleen tissue with ME2 antibody at 5 μg/mL.
Immunofluorescence of ME2 in human spleen tissue with ME2 antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
ME2 Antibody: ODS1, NAD-dependent malic enzyme, mitochondrial, Malic enzyme 2, NAD-ME
Application Note:
ME2 antibody can be used for detection of ME2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate (1). It is related to malic enzyme 1 (ME1), a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis (2). The expression of both malic enzymes is reciprocally regulated by p53; this regulation has been shown to modulate metabolism and senescence (3). Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy (4).
Background References:
  • Loeber G, Infante AA, Maurer-Fogy I, et al. Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli. J. Biol. Chem. 1991; 266:3016-21.
  • Gonzalez-Manchon C, Ferrer M, Ayuso MS, et al. Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver. Gene 1995;159:255-60.
  • Jiang P, Du W, Mancuso A, et al. Reciprocal regulation of p53 and malic enzymes modulates metabolism and senescence. Nature 2013; 493:689-83.
  • Lenzen KP, Heils A, Lorenz S, et al. Association analysis of malic acid enzyme 2 gene polymorphisms with idiopathic generalized epilepsy. Epilepsia 2005; 46:1637-41.
Buffer:
ME2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ME2 antibody was raised against a 17 amino acid peptide near the center of human ME2.

The immunogen is located within amino acids 340 - 390 of ME2.
NCBI Gene ID #:
4200
NCBI Official Name:
malic enzyme 2, NAD(+)-dependent, mitochondrial
NCBI Official Symbol:
ME2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 64 kDa

Observed: 60 kDa
Protein Accession #:
NP_002387
Protein GI Number:
4505145
Purification:
ME2 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis
SPECIFICITY:
ME2 antibody is human specific. At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is predicted not to cross-react with ME1.
Swissprot #:
P23368
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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