APOBEC3B Antibody

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ProSci
Product Code: PSI-7725
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7725-0.02mg0.02mg£150.00
Quantity:
PSI-7725-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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Western blot analysis of APOBEC3B in HepG2 cell lysate with APOBEC3B antibody at 1 μg/ml.
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Immunohistochemistry of APOBEC3B in human small intestine tissue with APOBEC3B antibody at 5 μg/mL.
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Immunofluorescence of APOBEC3B in human small intestine tissue with APOBEC3B antibody at 20 μg/mL.
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Western blot analysis of APOBEC3B in HepG2 cell lysate with APOBEC3B antibody at 1 μg/ml.
Immunohistochemistry of APOBEC3B in human small intestine tissue with APOBEC3B antibody at 5 μg/mL.
Immunofluorescence of APOBEC3B in human small intestine tissue with APOBEC3B antibody at 20 μg/mL.

Documents

Further Information

Additional Names:
APOBEC3B Antibody: A3B, ARP4, ARCD3, PHRBNL, APOBEC1L, bK150C2.2, DJ742C19.2Phorbolin-1-related protein, A3B
Application Note:
APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The Apolipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytosine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5? which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3). APOBEC3B has also been shown to be upregulated in several cancers; it is thought that APOBEC3B-catalyzed genomic uracil lesions are responsible for a large proportion of mutations in multiple distinct cancers (4).
Background References:
  • Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96.
  • Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5 restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
  • Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15.
  • Burns MB, Temiz NA, and Harris RS. Evidence for APOBEC3B mutagenesis in multiple human cancers. Nat. Genet. 2013; 45:977-83.
Buffer:
APOBEC3B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
APOBEC3B antibody was raised against a 14 amino acid peptide near the amino terminus of human APOBEC3B.

The immunogen is located within amino acids 20 - 70 of APOBEC3B.
NCBI Gene ID #:
9582
NCBI Official Name:
apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B
NCBI Official Symbol:
APOBEC3B
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 42 kDa

Observed: 48 kDa
Protein Accession #:
NP_004891
Protein GI Number:
393715117
Purification:
APOBEC3B antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
SPECIFICITY:
APOBEC3B antibody is human, mouse and rat reactive . At least three isoforms of APOBEC3B are known to exist; this antibody will detect all three. APOBEC3B antibody may cross-react with APOBEC3D.
Swissprot #:
Q9UH17
User NOte:
Optimal dilutions for each application to be determined by the researcher.

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