TRIM21 Antibody

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ProSci
Product Code: PSI-7729
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7729-0.02mg0.02mg£150.00
Quantity:
PSI-7729-0.1mg0.1mg£449.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

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<strong>Figure 1 Western Blot Validation in Human Lung Tissue Lysate in (Lane 1) the absence and (Lane 2) the presence of blocking peptide</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TRIM21, 7729 (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TRIM21, 7729 (0.5 μg/mL), TRIM21, 18-413 (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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<strong>Figure 3 Immunohistochemistry Validation of TRIM21 in Human Small Intestine Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TRIM21 antibody (7729) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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<strong>Figure 4 Immunofluorescence Validation of TRIM21 in Human Small Intestine Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human small intestine tissue labeling TRIM21 with 7729 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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<strong>Figure 1 Western Blot Validation in Human Lung Tissue Lysate in (Lane 1) the absence and (Lane 2) the presence of blocking peptide</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TRIM21, 7729 (0.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines</strong><br> Loading: 15 μg of lysates per lane. Antibodies: TRIM21, 7729 (0.5 μg/mL), TRIM21, 18-413 (2 μg/mL), and beta-actin (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Immunohistochemistry Validation of TRIM21 in Human Small Intestine Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TRIM21 antibody (7729) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 4 Immunofluorescence Validation of TRIM21 in Human Small Intestine Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human small intestine tissue labeling TRIM21 with 7729 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Documents

Further Information

Additional Names:
TRIM21 Antibody: SSA, RO52, SSA1, RNF81, Ro/SSA, E3 ubiquitin-protein ligase TRIM21, 52 kDa Ro protein, SS-A
Application Note:
WB: 0.5-2 μg/mL; IHC: 5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1). RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2). TRIM21 also acts as a cytosolic antibody receptor; detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3).
Background References:
  • Oke and Wahren-Herlenius. J. Autoimmun. 2012; 39:77-82.
  • Frank et al. Biochem. J. 1995; 305:359-62.
  • McEwan. Nat. Immunol. 14:327-36.
Buffer:
TRIM21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine (73%), Mouse (69%) and Rat (69%).
Immunogen:
Anti-TRIM21 antibody (7729) was raised against a peptide corresponding to 16 amino acids near the center of human TRIM21.

The immunogen is located within amino acids 290-340 of TRIM21.
ISOFORMS:
Human TRIM21 has 2 isoforms, including isoform 1 (475aa, 54kD) and isoform 2 (398aa, 45kD). Mouse TRIM21 has one isoform (470aa, 54kD) and Rat TRIM21 also has one isoform (471aa, 54kD). 7729 can detect human and possibly mouse and rat.
NCBI Gene ID #:
6737
NCBI Official Name:
tripartite motif containing 21
NCBI Official Symbol:
TRIM21
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 54kD

Observed: 54 kD
Protein Accession #:
NP_003132
Protein GI Number:
15208660
Purification:
TRIM21 antibody is affinity chromatography purified via peptide column.
Research Area:
Innate Immunity
SPECIFICITY:
TRIM21 antibody is human specific. This antibody is predicted to not cross-react with TRIM6.
Swissprot #:
P19474
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar TRIM21 expression profile in human cell lines detected by two independent anti-TRIM21 antibodies that recognize different epitopes, 7729 against central domain and 18-413 against recombinant fusion protein.  TRIM21 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

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