TET1 Antibody

Contact us
ProSci
Product Code: PSI-7733
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-7733-0.02mg0.02mg£150.00
Quantity:
PSI-7733-0.1mg0.1mg£449.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)

Images

1 / 8
<strong>Figure 1 WB Validation of TET1 in Human Cell Lines </strong><br> Loading: 15 μg of lysate Antibodies: TET1 7733, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
2 / 8
<strong> Figure 2 WB Validation of TET1 in Mouse Tissues </strong><br> Loading: 15 μg of lysate Antibodies: TET1 7733 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
3 / 8
<strong>Figure 3 WB Validation of TET1 in Rat Tissues</strong><br> Loading: 15 μg of lysate Antibodies: TET1 7733, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
4 / 8
<strong>Figure 4 Immunofluorescence Validation of TET1 in HeLa Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling TET1 with 7733 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
5 / 8
<strong>Figure 5 Immunofluorescence Validation of TET1 in Mouse Brain Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TET1 with 7733 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI antibody (blue).
6 / 8
<strong> Figure 6 Immunohistochemistry Validation of TET1 in Human Testis Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-TET1 antibody (7733) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
7 / 8
<strong>Figure 7 Immunohistochemistry Validation of TET1 in Mouse Kidney Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TET1 antibody (7733) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
8 / 8
<strong>Figure 8 Immunohistochemistry Validation of TET1 in Rat Liver Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-TET1 antibody (7733) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
❮❯

<strong>Figure 1 WB Validation of TET1 in Human Cell Lines </strong><br> Loading: 15 μg of lysate Antibodies: TET1 7733, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong> Figure 2 WB Validation of TET1 in Mouse Tissues </strong><br> Loading: 15 μg of lysate Antibodies: TET1 7733 , 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 WB Validation of TET1 in Rat Tissues</strong><br> Loading: 15 μg of lysate Antibodies: TET1 7733, 1 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunofluorescence Validation of TET1 in HeLa Cells</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed Hela cells labeling TET1 with 7733 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).
<strong>Figure 5 Immunofluorescence Validation of TET1 in Mouse Brain Tissue</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling TET1 with 7733 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI antibody (blue).
<strong> Figure 6 Immunohistochemistry Validation of TET1 in Human Testis Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-TET1 antibody (7733) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of TET1 in Mouse Kidney Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TET1 antibody (7733) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunohistochemistry Validation of TET1 in Rat Liver Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-TET1 antibody (7733) at 1 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Documents

Further Information

Additional Names:
TET1 Antibody: LCX, CXXC6, bA119F7.1, KIAA1676, LCX, Methylcytosine dioxygenase TET1, CXXC-type zinc finger protein 6
Application Note:
TET1 antibody can be used for detection of TET1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Background:
TET1, a member of the ten-eleven-translocation (TET) family of genes, was initially discovered as a fusion partner of MLL in acute myeloid leukemias containing the t(10;11)(q22;q23) (1). It is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine (2). TET1 expression is regulated during inflammation in both THP-1 and primary dendritic cells, and is a negative transcriptional regulator of IL-1beta following and inflammatory stimulus (3). Recent evidence has shown that TET1 is part of a signaling pathway that regulates breast cancer growth and metastasis (4).
Background References:
  • Lorsbach, et al. Leukemia 2003; 17:637-41.
  • Tahilani , et al. Science 2009; 324:930-5.
  • Neves-Costa, et al. Immunol. 2013; 54:264-70.
  • Sun, et al. Sci. USA 2013; 110:9920-5.
Buffer:
TET1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%), Pig: (88%)
Immunogen:
TET1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human TET1.

The immunogen is located within amino acids 2030 - 2080 of TET1.
Isoforms:
Human TET1 has one isoform (2136aa, 235kD). Mouse TET1 has one isoform (2007aa, 219kD) and Rat TET1 also has one isoform (2033aa, 223kD). 7733 can detect human, mouse and rat.
NCBI Gene ID #:
80312
NCBI Official Name:
tet methylcytosine dioxygenase 1
NCBI Official Symbol:
TET1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 235 kDa

Observed: 240 kDa
Protein Accession #:
NP_085128
Protein GI Number:
156139122
Purification:
TET1 antibody is affinity chromatography purified via peptide column.
Research Area:
Cancer, Cell Cycle
SPECIFICITY:
TET1 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with TET2 and TET3.
Swissprot #:
Q8NFU7
User NOte:
Optimal dilutions for each application to be determined by the researcher.

References

  • McLaughlin et al. Characterizing Requirements for Small Ubiquitin-like Modifier (SUMO) Modification and Binding on Base Excision Repair Activity of Thymine-DNA Glycosylase in Vivo. J Biol Chem. 2016;291(17):9014-24PMID: 26917720

Related Products

Product NameProduct CodeSupplier 
TET1 PeptidePSI-7733PProSci Summary Details