OLIG1 Antibody
| Code | Size | Price |
|---|
| PSI-7741-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7741-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
OLIG1 Antibody: BHLHB6, BHLHE21, BHLHB6, Oligodendrocyte transcription factor 1, Class B basic helix-loop-helix protein 6, Oligo1
Application Note:
OLIG1 antibody can be used for detection of OLIG1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The oligodendrocyte transcription factors 1 and 2 (OLIG1 and OLIG2, respectively) make up part of basic helix-loop-helix (bHLH) family of transcription factors that are specifically expressed in zones of the neuroepithelium from which oligodendrocyte precursors emerge (1). Both OLIG1 and OLIG2 genes are downstream targets of Sonic hedgehog and are expressed exclusively in the central nervous system (2). OLIG1 is first expressed in the dorsal portion of the p3 progenitor domain of the ventral neural tube while OLIG2 is first observed in the ventral most p3 domain (2). OLIG1 has been shown to be a SMAD cofactor involved in cell motility induced by transforming growth factor-beta (TGF-beta) (3).
Background References:
- Zhou Q, Wang S, and Anderson DJ. Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors. Neuron 2000; 25:331-43.
- Lu QR, Yuk D, Alberta JA, et al. Sonic Hedgehog-regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system. Neuron 2000; 25:317-29.
- Motizuki M, Isogaya K, Miyake K, et al. Oligodendrocyte transcription factor 1 (Olig1) is a Smad cofactor involved in cell motility induced by transforming growth factor-b. J. Biol. Chem. 2013; 288:18911-22.
Buffer:
OLIG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (83%), Mouse: (78%)
Immunogen:
OLIG1 antibody was raised against an 18 amino acid peptide near the amino terminus of human OLIG1.
The immunogen is located within amino acids 50 - 100 of OLIG1.
The immunogen is located within amino acids 50 - 100 of OLIG1.
NCBI Gene ID #:
116448
NCBI Official Name:
oligodendrocyte transcription factor 1
NCBI Official Symbol:
OLIG1
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 30 kDa
Observed: 29 kDa
Observed: 29 kDa
Protein Accession #:
NP_620450
Protein GI Number:
237757328
Purification:
OLIG1 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
OLIG1 antibody is human specific. It is predicted to not cross-react with other members of the OLIG family of proteins.
Swissprot #:
Q8TAK6
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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