OLIG2 Antibody
| Code | Size | Price |
|---|
| PSI-7743-0.02mg | 0.02mg | £150.00 |
Quantity:
| PSI-7743-0.1mg | 0.1mg | £449.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Applications:
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blot (WB)
Documents
Further Information
Additional Names:
OLIG2 Antibody: BHLHB1, OLIGO2, RACK17, PRKCBP2, bHLHe19, BHLHB1, BHLHE19, Oligodendrocyte transcription factor 2, Class B basic helix-loop-helix protein 1, Oligo2
Application Note:
OLIG2 antibody can be used for detection of OLIG2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Background:
The oligodendrocyte transcription factors 1 and 2 (OLIG1 and OLIG2, respectively) make up part of basic helix-loop-helix (bHLH) family of transcription factors that are specifically expressed in zones of the neuroepithelium from which oligodendrocyte precursors emerge (1). Both OLIG1 and OLIG2 genes are downstream targets of Sonic hedgehog and are expressed exclusively in the central nervous system (2). OLIG2 is first observed in the ventral most p3 progenitor domain of the ventral neural tube while OLIG1 is first expressed in the dorsal portion of the p3 domain (2). Mice overexpressing OLIG2 exhibit impaired potassium channel expression in neural progenitors and proliferation of these cells similar to that seen in Down Syndrome, suggesting that OLIG2 may play a role in this pathology (3).
Background References:
- Zhou Q, Wang S, and Anderson DJ. Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors. Neuron 2000; 25:331-43.
- Lu QR, Yuk D, Alberta JA, et al. Sonic Hedgehog-regulated oligodendrocyte lineage genes encoding bHLH proteins in the mammalian central nervous system. Neuron 2000; 25:317-29.
- Lu J, Lian G, Zhou H, et al. OLIG2 over-expression impairs proliferation of human Down syndrome neural progenitors. Hum. Mol. Genet. 2012; 21:2330-40.
Buffer:
OLIG2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen:
OLIG2 antibody was raised against a 15 amino acid peptide near the amino terminus of human OLIG2.
The immunogen is located within amino acids 80 - 130 of OLIG2.
The immunogen is located within amino acids 80 - 130 of OLIG2.
NCBI Gene ID #:
10215
NCBI Official Name:
oligodendrocyte lineage transcription factor 2
NCBI Official Symbol:
OLIG2
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 36 kDa
Observed: 37 kDa
Observed: 37 kDa
Protein Accession #:
NP_005797
Protein GI Number:
17978475
Purification:
OLIG2 antibody is affinity chromatography purified via peptide column.
Research Area:
Stem Cell
SPECIFICITY:
OLIG2 antibody is human, mouse and rat reactive. It is predicted to not cross-react with other members of the OLIG family of proteins. At least three isoforms of OLIG2 are known to exist; this antibody will detect all three isoforms.
Swissprot #:
Q13516
User NOte:
Optimal dilutions for each application to be determined by the researcher.
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