Uvula Tissue Slide (Benign)

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ProSci
Product Code: PSI-13-003
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-13-003-50uL50uL£541.00
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Overview

Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry (IHC)

Images

1 / 4
Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-003) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 4
Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-003) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
3 / 4
Immunofluorescence analysis of NIH/3T3 cells using PARP1 antibody (13-003) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of U2OS cells using PARP1 antibody (13-003) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-003) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37°C for 5 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using PARP1 antibody (13-003) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of NIH/3T3 cells using PARP1 antibody (13-003) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using PARP1 antibody (13-003) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
KIF3B, PARP, PPOL, ADPRT, ARTD1, ADPRT1, PARP-1, ADPRT 1, pADPRT-1
Application Note:
WB: 1:1000 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human PARP1 (NP_001609.2).
NCBI Gene ID #:
142
NCBI Official Name:
poly (ADP-ribose) polymerase 1
NCBI Official Symbol:
PARP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 116kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P09874
User NOte:
Optimal dilutions for each application to be determined by the researcher.